Evidence map›Paper›PMID 37089315›Full record

ArticleHeliyon2023

A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events.

Jason A Estep, Lu O Sun, Martin M Riccomagno

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jason A EstepCell, Molecular and Developmental Biology Program, Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA.
Lu O SunDepartment of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Martin M RiccomagnoCell, Molecular and Developmental Biology Program, Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA.

Funding

Molecular and genetic decoding of neuron-glial interactionsDP2MH129988 · NIMH · UT SOUTHWESTERN MEDICAL CENTER · PI SUN, LU · 2021 to 2024
$2.6M
Regulation of Neural Migration During Brain DevelopmentR01NS104026 · NINDS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI RICCOMAGNO, MARTIN MIGUEL · 2018 to 2022
$1.7M
Identification of a novel pathway that regulates optic nerve myelination and remyelinationR00EY029330 · NEI · UT SOUTHWESTERN MEDICAL CENTER · PI SUN, LU · 2020 to 2022
$884k
NEI NIH HHS R00 EY029330NIMH NIH HHS DP2 MH129988NINDS NIH HHS R01 NS104026
6 · The paper itself

Abstract

Integrin Adhesion Complexes (IACs) serve as links between the cytoskeleton and extracellular environment, acting as mechanosensing and signaling hubs. As such, IACs participate in many aspects of cellular motility, tissue morphogenesis, anchorage-dependent growth and cell survival. Focal Adhesion Kinase (FAK) has emerged as a critical organizer of IAC signaling events due to its early recruitment and diverse substrates, and thus has become a genetic and therapeutic target. Here we present the design and characterization of simple, reversible, and scalable Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells. These probes provide novel means to quantify IAC signaling, expanding on the currently available toolkit for interrogating FAK phosphorylation during diverse cellular processes.

Indexed as

Cell migrationCell motilityFocal adhesion kinaseFocal adhesionsIntegrinIntegrin adhesion complexesLuciferase fragment complementation assaySplit luciferase

Identifiers

PMID37089315
PMCPMC10119766

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.