ArticleThe Journal of biological chemistry2023
Augmin is a Ran-regulated spindle assembly factor.
Article in The Journal of biological chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Noncentrosomal Microtubule Assembly and Its Regulation.Cytoskeleton (Hoboken, N.J.) · 2026Review
- The γ-TuRC: mastermind of multipurpose actions at the centrosome.Cellular and molecular life sciences : CMLS · 2026Review
- The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts.Cytoskeleton (Hoboken, N.J.) · 2026Article
- How augmin establishes the angle of the microtubule branch site.Nature communications · 2025Article
- Conserved function of the HAUS6 calponin homology domain in anchoring augmin for microtubule branching.Nature communications · 2025Article
- Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC.Nature communications · 2025Article
- Phase separation of microtubule-binding proteins - implications for neuronal function and disease.Journal of cell science · 2024Review
- HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.Nature communications · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end.The EMBO journal · 2024Article
- Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos.Nature communications · 2024Article
- HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.bioRxiv : the preprint server for biology · 2023Article
- Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.Journal of cell science · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mitotic spindles are composed of microtubules (MTs) that must nucleate at the right place and time. Ran regulates this process by directly controlling the release of spindle assembly factors (SAFs) from nucleocytoplasmic shuttle proteins importin-αβ and subsequently forms a biochemical gradient of SAFs localized around chromosomes. The majority of spindle MTs are generated by branching MT nucleation, which has been shown to require an eight-subunit protein complex known as augmin. In Xenopus laevis, Ran can control branching through a canonical SAF, TPX2, which is nonessential in Drosophila melanogaster embryos and HeLa cells. Thus, how Ran regulates branching MT nucleation when TPX2 is not required remains unknown. Here, we use in vitro pulldowns and total internal reflection fluorescence microscopy to show that augmin is a Ran-regulated SAF. We demonstrate that augmin directly interacts with both importin-α and importin-β through two nuclear localization sequences on the Haus8 subunit, which overlap with the MT-binding site. Moreover, we show that Ran controls localization of augmin to MTs in both Xenopus egg extract and in vitro. Our results demonstrate that RanGTP directly regulates augmin, which establishes a new way by which Ran controls branching MT nucleation and spindle assembly both in the absence and presence of TPX2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.