ArticlePlant physiology2023
A multifunctional true caffeoyl coenzyme A O-methyltransferase enzyme participates in the biosynthesis of polymethoxylated flavones in citrus.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 43 citations in OpenAlex.
- Structural insights into a dual-substrate O-methyltransferase reveal the enzymatic mechanism of parallel paeonol biosynthesis.Nature communications · 2026Article
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- Metabolic Engineering for Biotechnological Production of Bioactive Methylated Flavonoids and Stilbenes: Progress and Perspectives.Applied biochemistry and biotechnology · 2026Review
- Enzymatic Modification of Flavonoids: Implications for Bioavailability, Bioactivity, and Therapeutic Potential Based on a Systematic Review and Bioinformatics Analysis.Antioxidants (Basel, Switzerland) · 2026Review
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- The Transcription Factors NFYA1 and GBF3 Jointly Regulate CHS2 to Promote Tangeretin Accumulation and Cold Tolerance in Citrus.Plant biotechnology journal · 2026Article
- MdCSN5-MdIAMT module promotes anthocyanin accumulation by regulating IAA homeostasis in apple.Horticulture research · 2026Article
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- Transcriptomic and Metabolomic Joint Analysis Revealing Different Metabolic Pathways and Genes Dynamically Regulating Bitter Gourd (Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Analysis of the Caffeoyl Coenzyme A-O-Methyltransferase (International journal of molecular sciences · 2025Article
- Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals Mechanism Underlying thePlants (Basel, Switzerland) · 2025Article
- Genome-wide characterization and expression profiling of theFrontiers in plant science · 2025Article
- Physiological and molecular regulatory mechanism of flavonoid metabolite biosynthesis during low temperature adaptation in Lavandula angustifolia Mill.BMC plant biology · 2024Article
- Genome-Wide Characterization ofGenes · 2024Article
- Genome-Wide Analysis of Caffeoyl-CoA-O-methyltransferase (CCoAOMT) Family Genes and the Roles ofPlants (Basel, Switzerland) · 2024Article
- Systematic Identification and Characterization ofInternational journal of molecular sciences · 2024Article
- Integrated Metabolomic-Transcriptomic Analyses of Flavonoid Accumulation in Citrus Fruit under Exogenous Melatonin Treatment.International journal of molecular sciences · 2024Article
- Article
- An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis.Nature communications · 2024Article
- Widely Targeted Metabolomics Analysis Reveals Metabolites Important for Antioxidant Properties and Quality Traits in Different Fruit Parts of Aurantii Fructus Immatures.Molecules (Basel, Switzerland) · 2024Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Polymethoxylated flavones (PMFs) have received extensive attention due to their abundant bioactivities. Citrus peels specifically accumulate abundant PMFs, and methylation modification is a key step in PMF biosynthesis; however, the function of reported O-methyltransferase (OMT) in citrus is insufficient to elucidate the complete methylation process of PMFs. In this study, we analyzed the accumulation pattern of PMFs in the flavedo of the sweet orange (Citrus sinensis) cultivar "Bingtangcheng" at different developmental stages. We found that accumulation of PMFs was completed at the early stage of fruit development (60-d after flowering). Furthermore, we characterized a true caffeoyl-CoA O-methyltransferase (named CsCCoAOMT1) from C. sinensis. Functional analysis in vitro showed that CsCCoAOMT1 preferred flavonoids to caffeoyl-CoA and esculetin. This enzyme efficiently methylated the 6-, 7- 8-, and 3'-OH of a wide array of flavonoids with vicinal hydroxyl groups with a strong preference for quercetin (flavonol) and flavones. The transient overexpression and virus-induced gene silencing experiments verified that CsCCoAOMT1 could promote the accumulation of PMFs in citrus. These results reveal the function of true CCoAOMTs and indicate that CsCCoAOMT1 is a highly efficient multifunctional O-methyltransferase involved in the biosynthesis of PMFs in citrus.
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