Evidence map›Paper›PMID 37085909›Full record

ArticleStem cell research & therapy2023

Metabolic profile of mesenchymal stromal cells and macrophages in the presence of polyethylene particles in a 3D model.

Victoria Teissier, Qi Gao, Huaishuang Shen, Jiannan Li, Xueping Li, Elijah Ejun Huang, Junichi Kushioka, Masakazu Toya, Masanori Tsubosaka, Hirohito Hirata and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
  3. Engineering the Immune Response to Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Victoria TeissierDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA. vic.teissier@gmail.com.ORCID 0000-0002-0048-5453
Qi GaoDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Huaishuang ShenDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Jiannan LiDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Xueping LiDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Elijah Ejun HuangDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Junichi KushiokaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Masakazu ToyaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Masanori TsubosakaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Hirohito HirataDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Hossein Vahid AlizadehDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Chima V MadukaInstitute for Quantitative Health Science and Engineering, Departments of Biomedical Engineering and Microbiology and Molecular Genetics, Michigan State University, Michigan, USA.
Christopher H ContagInstitute for Quantitative Health Science and Engineering, Departments of Biomedical Engineering and Microbiology and Molecular Genetics, Michigan State University, Michigan, USA.
Yunzhi Peter YangDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Ning ZhangDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Stuart B GoodmanDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA, USA. goodbone@stanford.edu.
Stanford University · USMichigan State University · USClinical Innovations (United States) · USStanford Medicine · US

Funding

Wear Particle Disease and NF-kappa B SignalingR01AR063717 · NIAMS · STANFORD UNIVERSITY · PI GOODMAN, STUART B · 2012 to 2021
$3.5M
Tissue Engineering Approaches for Improved Treatment of Early Stage Osteonecrosis of the HipR01AR072613 · NIAMS · STANFORD UNIVERSITY · PI GOODMAN, STUART B, YANG, YUNZHI · 2018 to 2022
$2.2M
Customized MSCs to Enhance Healing of Bone DefectsR01AR073145 · NIAMS · STANFORD UNIVERSITY · PI GOODMAN, STUART B · 2018 to 2021
$1.4M
NIAMS NIH HHS R01 AR063717NIAMS NIH HHS R01 AR072613NIAMS NIH HHS R01 AR073145
6 · The paper itself

Abstract

backgroundContinuous cross talk between MSCs and macrophages is integral to acute and chronic inflammation resulting from contaminated polyethylene particles (cPE); however, the effect of this inflammatory microenvironment on mitochondrial metabolism has not been fully elucidated. We hypothesized that (a) exposure to cPE leads to impaired mitochondrial metabolism and glycolytic reprogramming and (b) macrophages play a key role in this pathway.

methodsWe cultured MSCs with/without uncommitted M0 macrophages, with/without cPE in 3-dimensional gelatin methacrylate (3D GelMA) constructs/scaffolds. We evaluated mitochondrial function (membrane potential and reactive oxygen species-ROS production), metabolic pathways for adenosine triphosphate (ATP) production (glycolysis or oxidative phosphorylation) and response to stress mechanisms. We also studied macrophage polarization toward the pro-inflammatory M1 or the anti-inflammatory M2 phenotype and the osteogenic differentiation of MSCs.

resultsExposure to cPE impaired mitochondrial metabolism of MSCs; addition of M0 macrophages restored healthy mitochondrial function. Macrophages exposed to cPE-induced glycolytic reprogramming, but also initiated a response to this stress to restore mitochondrial biogenesis and homeostatic oxidative phosphorylation. Uncommitted M0 macrophages in coculture with MSC polarized to both M1 and M2 phenotypes. Osteogenesis was comparable among groups after 21 days.

conclusionThis work confirmed that cPE exposure triggers impaired mitochondrial metabolism and glycolytic reprogramming in a 3D coculture model of MSCs and macrophages and demonstrated that macrophages cocultured with MSCs undergo metabolic changes to maintain energy production and restore homeostatic metabolism.

Indexed as

Mesenchymal Stem CellsOsteogenesisMacrophagesMetabolomePolyethylenePolyethyleneMacrophageMetabolismMitochondriaMSCsPolyethylene particlesThree-dimensional model

Identifiers

PMID37085909
PMCPMC10122387
OpenAlexW4366679996

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.