ArticlePloS one2023
Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Ovarian Function, Hormonal, Metabolic, Oxidative Stress, and Gene Expression Biomarker Responses of Holstein Heifers Under Heat Stress Conditions to a Reduced Dose of Nanofabricated GnRH Analog Within the Ovsynch Protocol.Animals : an open access journal from MDPI · 2026Article
- Advanced three-dimensional culture technologies in reproductive medicine: A narrative review.International journal of reproductive biomedicine · 2026Review
- Effects of Liposome-Encapsulated α-Pinene on In Vitro Oocyte Maturation and Embryo Development in Bovine Species.Molecular reproduction and development · 2026Article
- Folliculogenesis: A Cellular Crosstalk Mechanism.Current issues in molecular biology · 2025Review
- Effect of Liquid Marble 3D Culture System on In Vitro Maturation and Embryo Development of Prepubertal Goat Oocytes.Animals : an open access journal from MDPI · 2025Article
- Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos.Heliyon · 2024Article
- Using Hybrid Coating to Fabricate Highly Stable and Expandable Transparent Liquid Marbles.ACS applied materials & interfaces · 2024Article
- Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis.Amino acids · 2024Article
- Recent advancements to increase success in assisted reproductive technologies in cattle.Animal reproduction · 2024Review
- 3D culture applied to reproduction in females: possibilities and perspectives.Animal reproduction · 2024Review
- Application of Enzyme-Linked Fluorescence Assay (ELFA) to Obtain In Vivo Matured Dog Oocytes through the Assessment of Progesterone Level.Animals : an open access journal from MDPI · 2023Article
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system.
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