Evidence map›Paper›PMID 37083339›Full record

ArticleACS infectious diseases2023

Genome-Wide Libraries for Protozoan Pathogen Drug Target Screening Using Yeast Surface Display.

Rhiannon Heslop, Mengjin Gao, Andressa Brito Lira, Tamara Sternlieb, Mira Loock, Sahil Rao Sanghi, Igor Cestari

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rhiannon HeslopInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.
Mengjin GaoInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.
Andressa Brito LiraInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.
Tamara SternliebInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.ORCID 0000-0002-1167-5367
Mira LoockInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.
Sahil Rao SanghiInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.
Igor CestariInstitute of Parasitology, McGill University, Ste Anne de Bellevue, Montreal, QC H9X 3V9, Canada.ORCID 0000-0003-3845-7535

Funding

CIHR PJT-175222
6 · The paper itself

Abstract

The lack of genetic tools to manipulate protozoan pathogens has limited the use of genome-wide approaches to identify drug or vaccine targets and understand these organisms' biology. We have developed an efficient method to construct genome-wide libraries for yeast surface display (YSD) and developed a YSD fitness screen (YSD-FS) to identify drug targets. We show the efficacy of our method by generating genome-wide libraries for

Indexed as

Trypanosoma brucei bruceiTrypanosoma cruziMetronidazoleNucleotidesSaccharomyces cerevisiaeMetronidazoleNucleotidesgenomic librarygiardiahigh-throughput sequencingmetronidazoletrypanosomayeast surface display

Identifiers

PMID37083339
PMCPMC10187560

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.