ArticleFrontiers in physiology2023
The bioenergetic "CK Clamp" technique detects substrate-specific changes in mitochondrial respiration and membrane potential during early VML injury pathology.
Article in Frontiers in physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Running-based rehabilitation does not improve muscle function after volumetric muscle loss despite improving hindlimb weight-bearing asymmetry in mice.Experimental physiology · 2026Article
- Loss of ovarian hormones modulates sex-specific metabolic adaptations to volumetric muscle loss.American journal of physiology. Cell physiology · 2026Article
- 17β-Estradiol mitigates ovariectomy-induced defects in mitochondrial bioenergetics and redox balance after VML injury in female mice.American journal of physiology. Cell physiology · 2026Article
- When rehabilitation is not enough, how targeting metabolism can overcome the limited plasticity of skeletal muscle after traumatic injury.Experimental physiology · 2026Review
- Neurometabolic substrate utilization governs oxidative phosphorylation conductance in cortex and hippocampus.Journal of neurophysiology · 2026Article
- Peripheral blood mononuclear cell mitochondrial bioenergetics are related to vascular endothelial function in young and older adults.American journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- Novel assessment of postprandial metabolism reveals sex-specific metabolic flexibility and lipid remodeling following volumetric muscle loss.American journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- Retrospective transcriptomic analysis indicates temporal dysregulation of mitochondrial genes and metabolic pathways after volumetric muscle loss injury.Physiological reports · 2025Article
- Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice.BMC musculoskeletal disorders · 2025Article
- Menstrual cycle influence on skeletal muscle mitochondrial respiration in humans.Physiological reports · 2025Article
- Acute mitochondrial reactive oxygen species emissions drive mitochondrial dysfunction after traumatic muscle injury in male mice.American journal of physiology. Cell physiology · 2025Article
- Low intensity, high frequency vibration training to improve musculoskeletal function in a mouse model of volumetric muscle loss.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Traumatic Skeletal Muscle Injury and Recovery.Advances in experimental medicine and biology · 2025Review
- Advancing β-adrenoreceptor agonism for recovery after volumetric muscle loss through regenerative rehabilitation and biomaterial delivery approaches.Regenerative biomaterials · 2025Article
- Limitations in metabolic plasticity after traumatic injury are only moderately exacerbated by physical activity restriction.npj metabolic health and disease · 2024Article
- Differential effects of Western diet and traumatic muscle injury on skeletal muscle metabolic regulation in male and female mice.Journal of cachexia, sarcopenia and muscle · 2023Article
- Compromised Muscle Properties in a Severe Hypophosphatasia Murine Model.International journal of molecular sciences · 2023Article
- Resistance wheel running improves contractile strength, but not metabolic capacity, in a murine model of volumetric muscle loss injury.Experimental physiology · 2023Article
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6 authors.
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Abstract
Volumetric muscle loss (VML) injuries are characterized by non-recoverable loss of tissue resulting in contractile and metabolic dysfunction. The characterization of metabolic dysfunction in volumetric muscle loss-injured muscle has been interpreted from permeabilized myofiber respiration experiments involving saturating ADP levels and non-physiologic ATP:ADP concentration ratios. The extent to which this testing condition obscures the analysis of mitochondrial (dys) function after volumetric muscle loss injury is unclear. An alternative approach is described that leverages the enzymatic reaction of creatine kinase and phosphocreatine to assess mitochondrial respiration and membrane potential at clamped physiologic ATP:ADP ratios, "CK Clamp." The objective of this study was to validate the CK Clamp in volumetric muscle loss-injured muscle and to detect differences that may exist between volumetric muscle loss-injured and uninjured muscles at 1, 3, 5, 7, 10, and 14 days post-injury. Volumetric muscle loss-injured muscle maintains bioenergetic features of the CK Clamp approach, i.e., mitochondrial respiration rate (JO
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