ArticleNature methods2023
Engineering of NEMO as calcium indicators with large dynamics and high sensitivity.
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Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 53 citations in OpenAlex.
- Noninvasive methods to monitor dynamic single-cell events.Trends in cell biology · 2026Review
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- Sensing and Reprogramming Surface Receptor Activation With Synthetic Transcriptional Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Linear ubiquitination of the NMDA receptor GluN2A subunit facilitates the GluN2B-to-GluN2A switch and synaptic maturation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- CaBLAM: a high-contrast bioluminescent CaNature methods · 2026Article
- Identifying 2PHDO as a SOCE Inhibitor from Chinese Herbal Extracts.Current pharmaceutical biotechnology · 2026Article
- Active Zone Maturation Controls Presynaptic Output and Release Mode and Is Regulated by Neuronal Activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025Article
- Genetically encoded sensors illuminateIBRO neuroscience reports · 2025Article
- Highly dynamic and sensitive NEMOer calcium indicators for imaging ER calcium signals in excitable cells.Nature communications · 2025Article
- A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.PLoS biology · 2025Article
- Advances in two-photon imaging for monitoring neural activity in behaving mice.Frontiers in neuroscience · 2025Review
- Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.Journal of cell science · 2025Review
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Hierarchical behavior control by a single class of interneurons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations.Biophysics reports · 2024Article
- Article
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
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Authors and funding
21 authors at 6 institutions in 3 countries.
Jia Li *Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.ORCID 0000-0003-1274-3867
Ziwei Shang *State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.
Jia-Hui Chen *Hefei National Research Center for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, and Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Wenjia Gu *Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Li Yao *State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.
Xin YangExercise Physiology and Neurobiology Laboratory, College of PE and Sports, Beijing Normal University, Beijing, China.
Xiaowen SunState Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.
Liuqing WangBeijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Tianlu WangInstitute of Biosciences and Technology, Texas A&M University, Houston, TX, USA.
Siyao LiuInstitute of Biosciences and Technology, Texas A&M University, Houston, TX, USA.
Jiajing LiBeijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Tingting HouState Key Laboratory of Membrane Biology College of Life Sciences, Peking University, Beijing, China.
Dajun XingState Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.ORCID 0000-0002-6953-1198
Donald L GillDepartment of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA.
Jiejie LiBeijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Shi-Qiang WangState Key Laboratory of Membrane Biology College of Life Sciences, Peking University, Beijing, China.
Lijuan HouExercise Physiology and Neurobiology Laboratory, College of PE and Sports, Beijing Normal University, Beijing, China.
Yubin ZhouInstitute of Biosciences and Technology, Texas A&M University, Houston, TX, USA. yubinzhou@tamu.edu.
Ai-Hui TangHefei National Research Center for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, and Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China. tangah@ustc.edu.cn.
Xiaohui ZhangState Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China. xhzhang@bnu.edu.cn.
Youjun WangBeijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China. wyoujun@bnu.edu.cn.ORCID 0000-0003-0961-1716
Beijing Normal University · CNTexas A&M University · USPeking University · CNUniversity of Science and Technology of China · CNMcGovern Institute for Brain Research · USPennsylvania State University · US
Funding
Understanding Store-Operated Calcium Signal TransductionR35GM131916 · NIGMS · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI GILL, DONALD L · 2019 to 2023
$2.9MChina Postdoctoral Science Foundation) 2021M703089Chinese Ministry of Science and Technology | Department of S and T for Social Development (Department of S&T for Social Development) 2019YFA0802104National Natural Science Foundation of China (National Science Foundation of China) 32171033National Natural Science Foundation of China (National Science Foundation of China) 91954205National Natural Science Foundation of China (National Science Foundation of China) 92254301NIGMS NIH HHS R35 GM131916
Abstract
Genetically encoded calcium indicators (GECIs) are indispensable tools for real-time monitoring of intracellular calcium signals and cellular activities in living organisms. Current GECIs face the challenge of suboptimal peak signal-to-baseline ratio (SBR) with limited resolution for reporting subtle calcium transients. We report herein the development of a suite of calcium sensors, designated NEMO, with fast kinetics and wide dynamic ranges (>100-fold). NEMO indicators report Ca
Indexed as
CalciumNeuronsAnimalsCalcium SignalingIndicators and ReagentsMammalsCalciumIndicators and Reagents
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