ArticleEnvironmental microbiology reports2023
Type-specific quantification of particulate methane monooxygenase gene of methane-oxidizing bacteria at the oxic-anoxic interface of a surface paddy soil by digital PCR.
Article in Environmental microbiology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Identification of microbial groups and functional genes associated with greenhouse gas emissions in prawn farms.Scientific reports · 2025Article
- Unelongated Stems are an Active Nitrogen-Fixing Site in Rice Stems Supported by Both Sugar and Methane Under Low Nitrogen Conditions.Rice (New York, N.Y.) · 2025Article
- Group-specific Quantification of mcrA genes of Methanogenic Archaea and "Candidatus Methanoperedens" by Digital PCR.Microbes and environments · 2025Article
- Expanding the Eco-collection of Methane-oxidizing Bacteria Inhabiting Rice Roots: Cultivation, Isolation, and Genomic Characterization of Isolates.Microbes and environments · 2025Article
- Ubiquity of methanogenic archaea in the trunk of coniferous and broadleaved tree species in a mountain forest.Antonie van Leeuwenhoek · 2024Article
- Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils.Microorganisms · 2023Article
- Type-specific quantification of particulate methane monooxygenase gene of methane-oxidizing bacteria at the oxic-anoxic interface of a surface paddy soil by digital PCR.Environmental microbiology reports · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aerobic methane-oxidizing bacteria (MOB) play an important role in mitigating methane emissions from paddy fields. In this study, we developed a differential quantification method for the copy number of pmoA genes of type Ia, Ib, and IIa MOB in paddy field soil using chip-based digital PCR. Three probes specific to the pmoA of type Ia, Ib, and IIa MOB worked well in digital PCR quantification when genomic DNA of MOB isolates and PCR-amplified DNA fragments of pmoA were examined as templates. When pmoA genes in the surface soil layer of a flooded paddy were quantified by digital PCR, the copy numbers of type Ia, Ib, and IIa MOB were 10
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