ArticleMicrobial cell factories2023
Optimizing yeast for high-level production of kaempferol and quercetin.
Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 36 citations in OpenAlex.
- Identification of potential flavonoids strongly correlated with bitterness and astringency in quinoa seedling vegetables via metabolomics and electronic tongue.Food chemistry: X · 2026Article
- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Quercetin: Mechanisms of Action, Clinical Evidence in Metabolic Syndrome, and Translational Opportunities in Food Preservation.Molecules (Basel, Switzerland) · 2026Review
- Integrated metabolomics and molecular docking reveal fermentation-dependent flavonoid transformation and bitterness attenuation inFood chemistry: X · 2026Article
- Metabolic Engineering Strategies for Flavonoid Biosynthesis in Saccharomyces cerevisiae: Current Advances and Future Perspectives.Biotechnology and bioengineering · 2026Review
- P450 Fusion Protein Expressed inMolecules (Basel, Switzerland) · 2026Article
- Enhanced gibberellic acid production in Fusarium fujikuroi through ARTP-EMS mutagenesis and fermentation optimization.Biotechnology letters · 2026Article
- Advanced applications of synthetic biology technology in biosynthesis of bioactive compounds from medicinal plants.Chinese herbal medicines · 2026Review
- Microbe-driven flavonoid biotransformation revealed by multi-omics analysis during Hua Feng Dan Yao Mu fermentation.Frontiers in microbiology · 2026Article
- Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering.Microbial cell factories · 2025Article
- De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield.Bioresources and bioprocessing · 2025Article
- Deciphering the endomicrobiome of Podophyllum hexandrum to reveal the endophytic bacterial-association of in-planta podophyllotoxin biosynthesis.World journal of microbiology & biotechnology · 2025Article
- Mechanism and application of yeast and its culture in regulating intestinal antioxidant defense in ruminants.Frontiers in veterinary science · 2025Review
- Biosynthesis of Apigenin Glucosides in EngineeredJournal of microbiology and biotechnology · 2024Article
- Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites.Molecules (Basel, Switzerland) · 2024Review
- Expanding flavone and flavonol production capabilities inFrontiers in bioengineering and biotechnology · 2023Article
- Engineered living materials for the conversion of a low-cost food-grade precursor to a high-value flavonoid.Frontiers in bioengineering and biotechnology · 2023Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTwo important flavonoids, kaempferol and quercetin possess remarkably potent biological impacts on human health. However, their structural complexity and low abundance in nature make both bulk chemical synthesis and extraction from native plants difficult. Therefore microbial production via heterologous expression of plant enzymes can be a safe and sustainable route for their production. Despite several attempts reported in microbial hosts, the production levels of kaempferol and quercetin still stay far behind compared to many other microbial-produced flavonoids.
resultsIn this study, Saccharomyces cerevisiae was engineered for high production of kaempferol and quercetin in minimal media from glucose. First, the kaempferol biosynthetic pathway was reconstructed via screening various F3H and FLS enzymes. In addition, we demonstrated that amplification of the rate-limiting enzyme AtFLS could reduce the dihydrokaempferol accumulation and improve kaempferol production. Increasing the availability of precursor malonyl-CoA further improved the production of kaempferol and quercetin. Furthermore, the highest amount of 956 mg L
conclusionsDe novo biosynthesis of kaempferol and quercetin in yeast was improved through increasing the upstream naringenin biosynthesis and debugging the flux-limiting enzymes together with fed-batch fermentations, up to gram per liter level. Our work provides a promising platform for sustainable and scalable production of kaempferol, quercetin and compounds derived thereof.
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