ReviewMarine life science & technology2021
Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms.
Review in Marine life science & technology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
48 citing papers in PubMed, 100 citations in OpenAlex.
- Unlocking Microbial Dark Matter: A Comprehensive Review of Isolation Technologies from Traditional Culturing to Single-Cell Technologies.Microorganisms · 2026Review
- Differential Induction and Resuscitation of the Viable but Non-Culturable (VBNC) State inMicroorganisms · 2026Article
- Repeat Prolonged Chlorination at Low Dose Induces Chlorine Tolerance inEnvironment & health (Washington, D.C.) · 2026Article
- Efficient resuscitation of early-stage viable but non-culturable cells ofApplied and environmental microbiology · 2026Article
- Growth-rate dependent response of mycobacteria to relief of inhibition by a bacteriostatic antibiotic.FEMS microbiology letters · 2026Article
- Benchmarking Cost-Effective DNA Extraction Kits for Diverse Metagenomic Samples.International journal of molecular sciences · 2025Article
- Probiotic Viability Reconsidered: Integrating VBNC Resuscitation and Culture-Independent Methods for Accurate Probiotic Enumeration.Microorganisms · 2025Review
- Darkness to Discovery: A Comprehensive Mini-Review on Culturable and Non-Culturable Microbial Diversity from Deep Sea.Microbial ecology · 2025Review
- Difficult-to-culture micro-organisms specifically isolated using the liquid-liquid co-culture method - towards the identification of bacterial species and metabolites supporting their growth.Microbiology (Reading, England) · 2025Article
- Insights into urinary catheter colonisation and polymicrobial biofilms of Candida- bacteria under flow condition.Scientific reports · 2025Article
- Thermal and storage stability of novel nutraceuticals combining potential probiotic Limosilactobacillus fermentum strains and freeze-dried jabuticaba [Myrciaria cauliflora (Mart.) O. Berg] peel.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Absolute quantification of VBNC state formation and resuscitation in alcohol-producingFrontiers in cellular and infection microbiology · 2025Article
- Article
- Rationally designed microbial communities in agri-food production systems: from research to market.ISME communications · 2025Article
- The role of virulence genes inFrontiers in cellular and infection microbiology · 2025Review
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Investigation of co-existing bacteria in platelets by employing long-term culturomics and metagenomics.Frontiers in cellular and infection microbiology · 2025Article
- Identification, Characterization, and Ultrastructure Analysis of the Phenol-DegradingMicroorganisms · 2024Article
- Persistence and Culturability ofAntibiotics (Basel, Switzerland) · 2024Article
- Multiple factors trigger the formation and resuscitation of the VBNC state in alcohol-producingApplied and environmental microbiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culturing has been the cornerstone of microbiology since Robert Koch first successfully cultured bacteria in the late nineteenth century. However, even today, the majority of microorganisms in the marine environment remain uncultivated. There are various explanations for the inability to culture bacteria in the laboratory, including lack of essential nutrients, osmotic support or incubation conditions, low growth rate, development of micro-colonies, and the presence of senescent or viable but nonculturable (VBNC) cells. In the marine environment, many bacteria have been associated with dormancy, as typified by the VBNC state. VBNC refers to a state where bacteria are metabolically active, but are no longer culturable on routine growth media. It is apparently a unique survival strategy that has been adopted by many microorganisms in response to harsh environmental conditions and the bacterial cells in the VBNC state may regain culturability under favorable conditions. The resuscitation of VBNC cells may well be an important way to cultivate the otherwise uncultured microorganisms in marine environments. Many resuscitation stimuli that promote the restoration of culturability have so far been identified; these include sodium pyruvate, quorum sensing autoinducers, resuscitation-promoting factors Rpfs and YeaZ, and catalase. In this review, we focus on the issues associated with bacterial culturability, the diversity of bacteria entering the VBNC state, mechanisms of induction into the VBNC state, resuscitation factors of VBNC cells and implications of VBNC resuscitation stimuli for cultivating these otherwise uncultured microorganisms. Bringing important microorganisms into culture is still important in the era of high-throughput sequencing as their ecological functions in the marine environment can often only be known through isolation and cultivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.