ArticleMarine life science & technology2021
Optimizing the hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) protocol for detection of microbes in sediments.
Article in Marine life science & technology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 16 citations in OpenAlex.
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- ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets.Nature protocols · 2024Review
- Thermophilic Hadarchaeota grow on long-chain alkanes in syntrophy with methanogens.Nature communications · 2024Article
- Eukaryotic Cell Capture by Amplified MagneticFrontiers in microbiology · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Fluorescence in situ hybridization (FISH) is a canonical tool commonly used in environmental microbiology research to visualize targeted cells. However, the problems of low signal intensity and false-positive signals impede its widespread application. Alternatively, the signal intensity can be amplified by incorporating Hybridization Chain Reaction (HCR) with FISH, while the specificity can be improved through protocol modification and proper counterstaining. Here we optimized the HCR-FISH protocol for studying microbes in environmental samples, particularly marine sediments. Firstly, five sets of HCR initiator/amplifier pairs were tested on the laboratory-cultured bacterium Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-021-00098-8.
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