Evidence map›Paper›PMID 37072830›Full record

ArticleAnnals of clinical microbiology and antimicrobials2023

Improvement in Neisseria gonorrhoeae culture rates by bedside inoculation and incubation at a clinic for sexually transmitted infections.

L M Brendefur Corwin, P Campbell, K Jakobsen, F Müller, X Lai, M Unemo, T M Leegaard, J Vildershøj Bjørnholt, A O Olsen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of clinical microbiology and antimicrobials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Observational
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

L M Brendefur CorwinDepartment of Microbiology, Oslo University Hospital, Oslo, Norway. libren@ous-hf.no.ORCID http://orcid.org/0000-0001-5108-3350
P CampbellInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.ORCID http://orcid.org/0000-0002-8959-4161
K JakobsenDepartment of Microbiology, Oslo University Hospital, Oslo, Norway.ORCID http://orcid.org/0000-0002-8327-3578
F MüllerDepartment of Microbiology, Oslo University Hospital, Oslo, Norway.ORCID http://orcid.org/0000-0001-8934-2784
X LaiDepartment of Biostatistics, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.ORCID http://orcid.org/0000-0002-7364-3993
M UnemoWHO Collaborating Centre for Gonorrhoea and other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.ORCID http://orcid.org/0000-0003-1710-2081
T M LeegaardInstitute of Clinical Medicine, University of Oslo, Oslo, Norway.ORCID http://orcid.org/0000-0002-5767-4992
J Vildershøj BjørnholtDepartment of Microbiology, Oslo University Hospital, Oslo, Norway.ORCID http://orcid.org/0000-0002-0079-8062
A O OlsenSection for Respiratory, Blood-borne and Sexually Transmitted Infections, Department of Infection Control and Vaccines, Norwegian Institute of Public Health, Oslo, Norway.ORCID http://orcid.org/0000-0003-3757-1619
Oslo University Hospital · NOUniversity of Oslo · NONorwegian Institute of Public Health · NOÖrebro University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCulture of Neisseria gonorrhoeae is essential for surveillance of complete antimicrobial susceptibility profiles. In 2014, the culture success rate of N. gonorrhoeae from samples taken at the clinic for sexually transmitted infections (STI clinic), Oslo University Hospital, Norway, was only 20%. The present study aimed to improve gonococcal culture rates using bedside inoculation of patient samples on gonococcal agar plates and incubation at the STI clinic.

methodsThis prospective quality improvement study was conducted by the STI clinic and the Department of Microbiology at Oslo University Hospital from May 2016 - October 2017. When culture of N. gonorrhoeae was clinically indicated, we introduced a parallel 'bedside culture' at the STI clinic and compared results with the standard culture at the microbiology department. Samples were taken from urethra, anorectum, pharynx and cervix. Culture rates were compared across symptomatic and asymptomatic anatomical sites.

resultsFrom 596 gonococcal-positive PCR samples, bedside culture had a significantly higher success rate of 57% compared to 41% with standard culture (p < 0.05). Overall, culture rate from symptomatic sites was 91% v. 45% from asymptomatic sites. The culture rates from different anatomical sites were as follows: urethra 93%, anorectum 64%, pharynx 28% and cervix 70%. Bedside culture significantly (p < 0.05) improved the culture rates for symptomatic urethral and asymptomatic pharyngeal samples.

conclusionsWhere feasible, bedside inoculation on gonococcal agar plates and incubation of samples from patients with gonorrhoea is recommended. This will improve the culture diagnostics and provide additional gonococcal isolates for antimicrobial resistance surveillance.

Indexed as

Anti-Infective AgentsGonorrheaSexually Transmitted DiseasesAgarAnti-Bacterial AgentsDrug Resistance, BacterialFemaleHumansMicrobial Sensitivity TestsNeisseria gonorrhoeaeProspective StudiesAgarAnti-Bacterial AgentsAnti-Infective AgentsAntibiotic resistanceCultureGonorrhoeaNeisseria gonorrhoeae

Identifiers

PMID37072830
PMCPMC10114361
OpenAlexW4366264524

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.