ArticleCell2023
Pyramidal neurons form active, transient, multilayered circuits perturbed by autism-associated mutations at the inception of neocortex.
Article in Cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Article
- Dissociating the Hallucinogenic and Neuroplastic Effects of Psilocybin.bioRxiv : the preprint server for biology · 2026Article
- Activity-dependent development of vocal circuits in the neonatal rodent forebrain.EMBO reports · 2026Article
- Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology.STAR protocols · 2026Article
- Defective ventral neurogenesis due to midfetal Chd8 mutation drives autistic-like behavior in mice.Nature communications · 2026Article
- ImmatureProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Inapparent maternal ZIKV infection impacts fetal brain development and postnatal behavior.PLoS pathogens · 2026Article
- Cadherin 11 regulates presynaptic vesicle trafficking and neuronal activity in autism-related brain circuit.Molecular psychiatry · 2026Article
- ImmaturebioRxiv : the preprint server for biology · 2025Article
- Research progress of single cell RNA sequencing in nervous system.Molecular biology reports · 2025Review
- Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development.Nature neuroscience · 2025Article
- Single-cell technology grows up: Leveraging high-resolution omics approaches to understand neurodevelopmental disorders.Current opinion in neurobiology · 2025Review
- Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations.Nature communications · 2025Article
- Three systems of circuit formation: assembly, updating and tuning.Nature reviews. Neuroscience · 2025Review
- Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex.Nature communications · 2025Article
- Regulation of neural stem cells by innervating neurons.Journal of neurochemistry · 2025Review
- Development of spontaneous and sensory evoked network activity in rodent cerebral cortexFrontiers in cellular neuroscience · 2025Review
- Embryonically active piriform cortex neurons promote intracortical recurrent connectivity during development.Neuron · 2024Article
- Network state transitions during cortical development.Nature reviews. Neuroscience · 2024Review
- Review
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Authors and funding
17 authors.
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Abstract
Cortical circuits are composed predominantly of pyramidal-to-pyramidal neuron connections, yet their assembly during embryonic development is not well understood. We show that mouse embryonic Rbp4-Cre cortical neurons, transcriptomically closest to layer 5 pyramidal neurons, display two phases of circuit assembly in vivo. At E14.5, they form a multi-layered circuit motif, composed of only embryonic near-projecting-type neurons. By E17.5, this transitions to a second motif involving all three embryonic types, analogous to the three adult layer 5 types. In vivo patch clamp recordings and two-photon calcium imaging of embryonic Rbp4-Cre neurons reveal active somas and neurites, tetrodotoxin-sensitive voltage-gated conductances, and functional glutamatergic synapses, from E14.5 onwards. Embryonic Rbp4-Cre neurons strongly express autism-associated genes and perturbing these genes interferes with the switch between the two motifs. Hence, pyramidal neurons form active, transient, multi-layered pyramidal-to-pyramidal circuits at the inception of neocortex, and studying these circuits could yield insights into the etiology of autism.
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