ArticleChemSusChem2023
Engineering a Carboxyl Methyltransferase for the Formation of a Furan-Based Bioplastic Precursor.
Article in ChemSusChem, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- A Comprehensive Review of Recent Advances in Sustainable Synthesis of Bio-Based Furanic Compounds Via Chemical, Biological, Chemobiological and Other Combination Approach in Benign Systems.Applied biochemistry and biotechnology · 2026Review
- O-methyltransferase CbzMT catalyzes iterative 3,4-dimethylations for carbazomycin biosynthesis.Engineering microbiology · 2024Article
- Engineering a Carboxyl Methyltransferase for the Formation of a Furan-Based Bioplastic Precursor.ChemSusChem · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
FtpM from Aspergillus fumigatus was the first carboxyl methyltransferase reported to catalyse the dimethylation of dicarboxylic acids. Here the creation of mutant R166M that can catalyse the quantitative conversion of bio-derived 2,5-furandicarboxylic acid (FDCA) to its dimethyl ester (FDME), a bioplastics precursor, was reported. Wild type FtpM gave low conversion due to its reduced catalytic efficiency for the second methylation step. An AlphaFold 2 model revealed a highly electropositive active site, due to the presence of 4 arginine residues, postulated to favour the binding of the dicarboxylic acid over the intermediate monoester. The R166M mutation improved both binding and turnover of the monoester to permit near quantitative conversion to the target dimethyl ester product. The mutant also had improved activity for other diacids and a range of monoacids. R166M was incorporated into 2 multienzyme cascades for the synthesis of the bioplastics precursor FDME from bioderived 5-hydroxymethylfurfural (HMF) as well as from poly(ethylene furanoate) (PEF) plastic, demonstrating the potential to recycle waste plastic.
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Registered trials
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