Evidence map›Paper›PMID 37066408›Full record

ArticlebioRxiv : the preprint server for biology2023

Monosynaptic inputs to ventral tegmental area glutamate and GABA co-transmitting neurons.

Emily D Prévost, Alysabeth Phillips, Kristoffer Lauridsen, Gunnar Enserro, Bodhi Rubinstein, Daniel Alas, Dillon J McGovern, Annie Ly, Makaila Banks, Connor McNulty and 5 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Emily D PrévostDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Alysabeth PhillipsDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Kristoffer LauridsenDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Gunnar EnserroDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Bodhi RubinsteinDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Daniel AlasDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Dillon J McGovernDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Annie LyDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Makaila BanksDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Connor McNultyDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
Yoon Seok KimDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
Lief E FennoDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
Charu RamakrishnanDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
Karl DeisserothDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
David H RootDepartment of Psychology and Neuroscience, University of Colorado Boulder, 2860 Wilderness Pl, Boulder, CO 80301.
University of Colorado Boulder · USStanford University · USHoward Hughes Medical Institute · USThe University of Texas at Austin · US

Funding

Training CoreP50DA042012 · NIDA · STANFORD UNIVERSITY · PI Sean Albert Quirin · 2017 to 2026
$27.1M
Genetic dissection of ventral tegmental area glutamate and GABA neurons in reward and aversionR01DA047443 · NIDA · UNIVERSITY OF COLORADO · PI ROOT, DAVID H · 2020 to 2024
$1.7M
Opera Phenix High Throughput and High Content Confocal MicroscopeS10OD025072 · OD · UNIVERSITY OF COLORADO · PI LIU, XUEDONG · 2018 to 2018
$1.1M
Balancing excitatory and inhibitory LHb signaling underlying stressF31MH125569 · NIMH · UNIVERSITY OF COLORADO · PI MCGOVERN, DILLON · 2021 to 2022
$78k
NIDA NIH HHS P50 DA042012NIDA NIH HHS R01 DA047443NIH HHS S10 OD025072NIMH NIH HHS F31 MH125569
6 · The paper itself

Abstract

A unique population of ventral tegmental area (VTA) neurons co-transmits glutamate and GABA as well as functionally signals rewarding and aversive outcomes. However, the circuit inputs to VTA VGluT2+VGaT+ neurons are unknown, limiting our understanding of the functional capabilities of these neurons. To identify the inputs to VTA VGluT2+VGaT+ neurons, we coupled monosynaptic rabies tracing with intersectional genetic targeting of VTA VGluT2+VGaT+ neurons in mice. We found that VTA VGluT2+VGaT+ neurons received diverse brain-wide inputs. The largest numbers of monosynaptic inputs to VTA VGluT2+VGaT+ neurons were from superior colliculus, lateral hypothalamus, midbrain reticular nucleus, and periaqueductal gray, whereas the densest inputs relative to brain region volume were from dorsal raphe nucleus, lateral habenula, and ventral tegmental area. Based on these and prior data, we hypothesized that lateral hypothalamus and superior colliculus inputs were glutamatergic neurons. Optical activation of glutamatergic lateral hypothalamus neurons robustly activated VTA VGluT2+VGaT+ neurons regardless of stimulation frequency and resulted in flee-like ambulatory behavior. In contrast, optical activation of glutamatergic superior colliculus neurons activated VTA VGluT2+VGaT+ neurons for a brief period of time at high stimulation frequency and resulted in head rotation and arrested ambulatory behavior (freezing). For both pathways, behaviors induced by stimulation were uncorrelated with VTA VGluT2+VGaT+ neuron activity. However, stimulation of glutamatergic lateral hypothalamus neurons, but not glutamatergic superior colliculus neurons, was associated with VTA VGluT2+VGaT+ footshock-induced activity. We interpret these results such that inputs to VTA VGluT2+VGaT+ neurons may integrate diverse signals related to the detection and processing of motivationally-salient outcomes. Further, VTA VGluT2+VGaT+ neurons may signal threat-related outcomes, possibly via input from lateral hypothalamus glutamate neurons, but not threat-induced behavioral kinematics.

Indexed as

co-transmitvesicular glutamate transporter 2VTA VGluT2 neurons

Identifiers

PMID37066408
PMCPMC10104150
OpenAlexW4362704029

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.