Evidence map›Paper›PMID 37066160›Full record

ArticlebioRxiv : the preprint server for biology2024

Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysis.

Caroline A McCormick, Stuart Akeson, Sepideh Tavakoli, Dylan Bloch, Isabel N Klink, Miten Jain, Sara H Rouhanifard

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Caroline A McCormickDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0009-0000-0670-6707
Stuart AkesonDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0009-0008-8008-2486
Sepideh TavakoliDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0000-0002-0802-7051
Dylan BlochDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0000-0002-5382-5765
Isabel N KlinkDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.
Miten JainDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0000-0002-4571-3982
Sara H RouhanifardDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, United States.ORCID 0000-0002-6991-4877
Northeastern University · US

Funding

Technology Development Coordinating CenterU24HG011735 · NHGRI · JACKSON LABORATORY · PI Mark D ADAMS · 2021 to 2026
$8.1M
Synthetic mRNA Control Set for Nanopore-Based Pseudouridine Modification Profiling in Human TranscriptomesR01HG012856 · NHGRI · NORTHEASTERN UNIVERSITY · PI Sara Hakim Rouhanifard · 2023 to 2026
$3.4M
Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptasesR01HG011087 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2020 to 2023
$3.0M
NHGRI NIH HHS R01 HG011087NHGRI NIH HHS R01 HG012856NHGRI NIH HHS U24 HG011735
6 · The paper itself

Abstract

Nanopore direct RNA sequencing (DRS) enables measurements of RNA modifications. Modification-free transcripts are a practical and targeted control for DRS, providing a baseline measurement for canonical nucleotides within a matched and biologically derived sequence context. However, these controls can be challenging to generate and carry nanopore-specific nuances that can impact analysis. We produced DRS datasets using modification-free transcripts from

Indexed as

BioinformaticsTranscriptomics

Identifiers

PMID37066160
PMCPMC10104151
OpenAlexW4362698352

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.