Evidence map›Paper›PMID 37064525›Full record

ArticleArXiv2023

Fluorescence Microscopy: a statistics-optics perspective.

Mohamadreza Fazel, Kristin S Grussmayer, Boris Ferdman, Aleksandra Radenovic, Yoav Shechtman, Jörg Enderlein, Steve Pressé

Abstract readPreprint
PubMed Full text at PMC
In one paragraph

Article in ArXiv, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mohamadreza FazelDepartment of Physics, Arizona State University, Tempe, Arizona, USA.
Kristin S GrussmayerDepartment of Bionanoscience, Faculty of Applied Science and Kavli Institute for Nanoscience, Delft University of Technology, Delft, Netherlands.
Boris FerdmanRussel Berrie Nanotechnology Institute and Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.
Aleksandra RadenovicLaboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland.
Yoav ShechtmanRussel Berrie Nanotechnology Institute and Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.
Jörg EnderleinIII. Institute of Physics - Biophysics, Georg August University, Göttingen, Germany.
Steve PresséDepartment of Physics, Arizona State University, Tempe, Arizona, USA.

Funding

Toward high spatiotemporal resolution models of single molecules for in vivo applicationsR35GM148237 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI Steve Presse · 2023 to 2026
$1.7M
A Bayesian nonparametric approach to superresolved tracking of multiple molecules inside living cellsR01GM130745 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI PRESSE, STEVE · 2019 to 2023
$1.5M
Theoretical Models of Single Molecule Dynamics from Minimal Photon NumbersR01GM134426 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI PRESSE, STEVE · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM130745NIGMS NIH HHS R01 GM134426NIGMS NIH HHS R35 GM148237
6 · The paper itself

Abstract

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or smaller than light's wavelength. Here we review the optics responsible for generating fluorescent images, fluorophore properties, microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement.

Identifiers

PMID37064525
PMCPMC10104198

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.