Evidence map›Paper›PMID 37062934›Full record

ArticleAnimal models and experimental medicine2023

Screening for urinary markers predicting hematopoietic stem cell injury induced by busulfan using genetically diverse mice.

Yuhang Sun, Bowen Guan, Xing Liu, Lingyan Zhang, Xinpei Wang, Aimin Meng, Ran Gao

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal models and experimental medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yuhang SunNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.ORCID 0000-0002-5296-8377
Bowen GuanNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Xing LiuNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Lingyan ZhangNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Xinpei WangNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Aimin MengNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Ran GaoNational Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Center of Technology Innovation for Animal Model, Beijing, China.
Chinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBusulfan (BU) is an alkylating agent used as a conditioning agent prior to hematopoietic stem cell (HSC) transplantation as it is known to be cytotoxic to host hematopoietic stem and progenitor cells. The susceptibility of HSCs to BU injury plays an important role in the myeloablative efficacy of BU. Different susceptibilities were demonstrated in genetically diverse (GD) mice in our preliminary research.

methodsThree strains of GD mice with different susceptibilities to BU-induced HSC injury were used for screening biological markers of HSC injury susceptibility in urine. The urine proteins were analyzed using liquid chromatography coupled with tandem mass spectrometry to screen for differentially expressed proteins. Screening for possible biomarkers based on differences in protein expression abundance was validated using enzyme-linked immunoassay (ELISA).

resultsFunctional analysis showed that the differential proteins were all involved in a series of biological pathways related to cellular senescence, apoptosis, and angiogenesis; whereas the differential proteins of the high-susceptible strain were enriched for the regulation of bone marrow microenvironment pathways, those of low-susceptible strain were enriched for the proapoptotic effect of GTPase pathways. Based on protein abundance differences, several urinary proteins that may be indicative of susceptibility were screened, and ELISA validation results showed that angiotensin-converting enzyme may be a potential biomarker predicting HSC susceptibility for BU conditioning.

conclusionsThis study indicates that urinary protein levels can reflect differences in susceptibility to BU-induced HSC injury. Using GD mice to construct genetic difference models will provide preclinical data for screening BU-related biological markers.

Indexed as

BusulfanHematopoietic Stem Cell TransplantationAlkylating AgentsAnimalsHematopoietic Stem CellsMiceTandem Mass SpectrometryAlkylating AgentsBusulfanangiotensin I converting enzymebusulfanhematopoietic stem cell injuryinbred genetic diversity miceurinary marker

Identifiers

PMID37062934
PMCPMC10158946
OpenAlexW4366083429

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.