ArticleNature communications2023
Next-generation large-scale binary protein interaction network for Drosophila melanogaster.
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Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
- Protein Stability, Turnover Kinetics, and Abundance Constrain the Scaling of Protein Interaction Networks.bioRxiv : the preprint server for biology · 2026Article
- FlyPredictome: A structural atlas of predicted protein-protein interactions inbioRxiv : the preprint server for biology · 2026Article
- Integrative phosphoproteomic network analysis identifies CAMK2D as a shared regulator of TPD52 family proteins in cancer.Protoplasma · 2026Article
- Comparative transcriptomic profiling reveals metabolic and regulatory features of Drosophila melanogaster S2 cells relative to newly hatched larval tissues.Genes & genomics · 2026Article
- Sarcopenia promotes tumorigenesis by disrupting NOTCH-SDC2-regulated biogenesis of muscle-derived extracellular vesicles.Nature communications · 2026Article
- TF2TG: an online resource mining the potential gene targets of transcription factors in Drosophila.Genetics · 2026Article
- Inflammaging and osteoarthritis - therapeutic opportunities.Frontiers in immunology · 2026Review
- Towards a phylogenetically informed approach to solving protein-protein interactions.Biochemical Society transactions · 2025Review
- Exercise suppresses DEAF1 to normalize mTORC1 activity and reverse muscle aging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cryptochrome interaction networks across different tissues in Drosophila melanogaster.Biology direct · 2025Article
- A Structure-Guided Kinase-Transcription Factor Interactome Atlas Reveals Docking Landscapes of the Kinome.bioRxiv : the preprint server for biology · 2025Article
- Male sterility in Drosophila mojavensis × D. arizonae hybrids is not caused by gene movements in the Y chromosome.Evolution; international journal of organic evolution · 2025Article
- Decoding ATXN2 Phosphocode: Structural Insights and Therapeutic Opportunities in Disease.The protein journal · 2025Review
- Transcriptional regulation of autophagy in skeletal muscle stem cells.Disease models & mechanisms · 2025Review
- Simplicity within biological complexity.Bioinformatics advances · 2025Article
- Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression.PLoS biology · 2025Article
- FlyPhoneDB2: A computational framework for analyzing cell-cell communication inComputational and structural biotechnology journal · 2025Article
- Article
- Transcription Factor Deformed Wings Is an Atg8a-Interacting Protein That Regulates Autophagy.Cells · 2024Article
- Accurate and sensitive interactome profiling using a quantitative protein-fragment complementation assay.Cell reports methods · 2024Article
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Authors and funding
43 authors at 9 institutions in 4 countries.
Hong-Wen Tang *Department of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0002-8347-9891
Kerstin Spirohn *Department of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0002-2071-1606
Yanhui HuDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Tong HaoDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0001-7908-3256
István A KovácsCenter for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA.ORCID 0000-0002-6890-6277
Yue GaoDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Richard BinariDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Donghui Yang-ZhouDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Kenneth H WanBerkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, 94720, USA.ORCID 0000-0002-9203-1909
Joel S BaderDepartment of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.ORCID 0000-0002-6020-4625
Dawit BalchaDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Wenting BianDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Benjamin W BoothBerkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, 94720, USA.
Atina G CotéCenter for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA.
Steffi de RouckCytokine Receptor Lab, VIB Center for Medical Biotechnology, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
Alice DesbuleuxDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Kah Yong GohProgram in Cancer and Stem Cell Biology, Duke-NUS Medical School, 8 College Road, Singapore, 169857, Singapore.
Dae-Kyum KimDepartment of Cancer Genetics and Genomics, Roswell Park Comprehensive Cancer Center, 665 Elm St., Buffalo, NY, 14203, USA.
Jennifer J KnappDonnelly Centre for Cellular and Biomolecular Research and Department of Molecular Genetics, University of Toronto, 160 College St, Toronto, ON, M5S 3E1, Canada.ORCID 0000-0003-3347-4686
Wen Xing LeeProgram in Cancer and Stem Cell Biology, Duke-NUS Medical School, 8 College Road, Singapore, 169857, Singapore.
Irma LemmensCytokine Receptor Lab, VIB Center for Medical Biotechnology, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
Cathleen LiDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Mian LiDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Roujia LiCenter for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA.
Hyobin Julianne LimDepartment of Cancer Genetics and Genomics, Roswell Park Comprehensive Cancer Center, 665 Elm St., Buffalo, NY, 14203, USA.
Yifang LiuDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Katja LuckDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0003-2336-9225
Dylan MarkeyDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Carl PollisDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0001-6453-0762
Sudharshan RangarajanDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Jonathan RodigerDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0002-1540-5030
Sadie SchlabachDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Yun ShenDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Dayag SheykhkarimliCenter for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA.ORCID 0000-0001-8415-6659
Bridget TeeKingDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Frederick P RothCenter for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA.ORCID 0000-0002-6628-649X
Jan TavernierCytokine Receptor Lab, VIB Center for Medical Biotechnology, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
Michael A CalderwoodDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0001-6475-1418
David E HillDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.ORCID 0000-0001-5192-0921
Susan E CelnikerBerkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, 94720, USA. celniker@fruitfly.org.
Marc VidalDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. marc_vidal@dfci.harvard.edu.
Norbert PerrimonDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. perrimon@genetics.med.harvard.edu.ORCID 0000-0001-7542-472X
Stephanie E MohrDepartment of Genetics, Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. stephanie_mohr@hms.harvard.edu.ORCID 0000-0001-9639-7708
Harvard University · USUniversity of Toronto · CALawrence Berkeley National Laboratory · USVIB-UGent Center for Medical Biotechnology · BEDuke-NUS Medical School · SGHoward Hughes Medical Institute · USRoswell Park Comprehensive Cancer Center · USJohns Hopkins University · USNorthwestern University · US
Funding
Characterization of the Insulin to Autophagy Pathway in MusclesR01AR057352 · NIAMS · HARVARD MEDICAL SCHOOL · PI NORBERT PERRIMON · 2010 to 2026
$8.2MLarge-Scale High-Confidence Binary Protein Interaction Network for DrosophilaR01HG007118 · NHGRI · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2013 to 2017
$4.3MHoward Hughes Medical InstituteNHGRI NIH HHS R01 HG007118NIAMS NIH HHS R01 AR057352
Abstract
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of > 10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of data from FlyBi and previous PPI studies using an orthogonal assay allows for normalization of data quality; subsequent integration of FlyBi and previous data results in an expanded binary Drosophila reference interaction network, DroRI, comprising 17,232 interactions among 6511 proteins. We use FlyBi data to generate an autophagy network, then validate in vivo using autophagy-related assays. The deformed wings (dwg) gene encodes a protein that is both a regulator and a target of autophagy. Altogether, these resources provide a foundation for building new hypotheses regarding protein networks and function.
Indexed as
Drosophila ProteinsProtein Interaction MapsAnimalsDrosophilaDrosophila melanogasterProtein Interaction MappingSaccharomyces cerevisiaeTwo-Hybrid System TechniquesDrosophila Proteins
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