ArticleFunctional & integrative genomics2023
RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes.
Article in Functional & integrative genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 287 papers.
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Who cites it
287 citing papers in PubMed.
- Selection and Validation of qRT-PCR Reference Genes inCurrent issues in molecular biology · 2026Article
- Identification and Validation of Reliable Reference Genes for Gene Expression Studies in Postharvest Atemoya Pulp (International journal of molecular sciences · 2026Article
- Concurrent Alterations in Selected mRNAs and miR-30 Family Members in Canine Osteoarthritic Joint Tissues.Biomedicines · 2026Article
- Resistance Exercise Training Selectively Modulates Tumor Suppressor and Muscle-Regulatory MicroRNAs in Breast Cancer Survivors and Healthy Postmenopausal Women: An Exploratory Study.International journal of molecular sciences · 2026Article
- Selection and evaluation of reference genes for RT-qPCR normalization across developmental stages and tissues in Dermestes maculatus (Coleoptera: Dermestidae).Journal of insect science (Online) · 2026Article
- Unidirectional porous beta-tricalcium phosphate supports three-dimensional osteogenic differentiation of human iPS cells without surface coating.Bone reports · 2026Article
- Differential Serum MicroRNA Profiling in Benign and Malignant Ovarian Tumours: An Exploratory Study Identifying hsa-miR-200c-3p as a Candidate Diagnostic Biomarker.Diagnostics (Basel, Switzerland) · 2026Article
- Article
- Screening of Stable Reference Genes inVeterinary sciences · 2026Article
- A clinical and experimental investigation of liraglutide effects on the brain-kidney axis.Science advances · 2026Article
- Gene Expression-Based Classification of European Seabass Larval Batches According to Saddleback Syndrome Incidence Using Machine Learning.Animals : an open access journal from MDPI · 2026Article
- Differences in mRNA expression of neuroinflammation-related genes in the temporal lobe of patients with drug-resistant focal epilepsy.Epileptic disorders : international epilepsy journal with videotape · 2026Article
- Establishing a biomarker set for the diagnosis of apple replant disease using qPCR.Horticulture research · 2026Article
- Reference gene selection for gene expression analysis in Coffea arabica L. under ABA and gibberellin treatments.Molecular biology reports · 2026Article
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Tissue-specific molecular responses to salinomycin and their modulation by ionophore-antibiotic interactions in turkeys.Poultry science · 2026Article
- Disruption of SlCNGC18 enhances Cucumber mosaic virus resistance and drought tolerance in tomato (Solanum lycopersicum L. cv. Micro-Tom).BMC plant biology · 2026Article
- Screening of Reference Gene for RT-qPCR inInternational journal of molecular sciences · 2026Article
- Reference Genes for Circadian Profiling of Core Clock Genes in the Blood of Obstructive Sleep Apnea Patients.Biomolecules · 2026Article
- Effects of Fluoride and 8:2 FTOH on β-Cell Calcium Signaling and Insulin Homeostasis: An Exploratory Study.Metabolites · 2026Article
227 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although many genes may serve as reference genes, they may cause different expression patterns by selecting different reference genes because no single gene is expressed consistently in all tested tissues of an organism under all environmental and developmental conditions. Thus, it is becoming increasingly important and necessary to identify suitable reference genes before performing gene expression analysis. Currently, there are several computational tools available for evaluating the stability of candidate reference genes. These tools are based on different statistical algorithms and may produce different rankings in stability within the same reference gene study. To date, the RefFinder is the only web-based tool available for comparing and evaluating housekeeping genes as candidates to be reference genes. In this tool, we integrated the four currently available computational programs (geNorm, NormFinder, BestKeeper, and the comparative ΔCt method) into a web-based tool for evaluating the stability and reliability of reference genes. According to the gene stability rankings derived from the four programs, we assigned an appropriate weight to each gene and calculated the geometric mean of weights for the final rankings. Aside from the overall ranking, a single program or combination of the four programs can be selected for evaluating the ranking of candidate reference genes. This tool has been widely used and validated by many research laboratories around the world. You may use this tool at http://www.heartcure.com.au/reffinder/ or https://blooge.cn/RefFinder/ . You can also download this algorithm program from https://github.com/fulxie/RefFinder and setup on your own computer. RefFinder is developed by PHP. Users can deploy it to a Php-based server (Apache + PHP) and run it.
Indexed as
Identifiers
37060478What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.