Evidence map›Paper›PMID 37060465›Full record

ArticleApplied microbiology and biotechnology2023

Detection of viral antibodies in camel sera using magnetic particle spectroscopy.

Bernhard Friedrich, Patrick Vogel, Martin A Rückert, Stefan Lyer, Johanna Günther, Ulrich Wernery, Sunitha Joseph, Judith Müller, Volker C Behr, Christoph Alexiou and 1 more

Abstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bernhard FriedrichDepartment of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany.
Patrick VogelDepartment of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
Martin A RückertDepartment of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
Stefan LyerDepartment of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany.
Johanna GüntherDepartment of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
Ulrich WerneryCentral Veterinary Research Laboratory, P.O. Box 597, Dubai, UAE.
Sunitha JosephCentral Veterinary Research Laboratory, P.O. Box 597, Dubai, UAE.
Judith MüllerGeneratio - Center for Animal Genetics, Heidelberg, Germany.
Volker C BehrDepartment of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
Christoph AlexiouDepartment of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany.
Rainer TietzeDepartment of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany. rainer.tietze@uk-erlangen.de.ORCID http://orcid.org/0000-0001-9306-633X

Funding

Deutsche Forschungsgemeinschaft AL 552/20-1Deutsche Forschungsgemeinschaft BE 5293/1-2Deutsche Forschungsgemeinschaft VO 2288/1-1Deutsche Forschungsgemeinschaft VO 2288/3-1
6 · The paper itself

Abstract

Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φ

Indexed as

COVID-19Middle East Respiratory Syndrome CoronavirusAnimalsAntibodies, ViralCamelusMagnetic PhenomenaRNA, ViralSARS-CoV-2Spectrum AnalysisAntibodies, ViralRNA, ViralAntibody detectionCamelsCOMPASSMNPsMPSRapid bioassaySARS-Cov-2

Identifiers

PMID37060465
PMCPMC10104765

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.