Evidence map›Paper›PMID 37058406›Full record

ArticleCell reports2023

Variant enterovirus A71 found in immune-suppressed patient binds to heparan sulfate and exhibits neurotropism in B-cell-depleted mice.

Kuo-Feng Weng, Han Kang Tee, Eirini D Tseligka, Valeria Cagno, Gregory Mathez, Stéphane Rosset, Claude M Nagamine, Peter Sarnow, Karla Kirkegaard, Caroline Tapparel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Serial passagingFrontiers in cellular and infection microbiology · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Kuo-Feng WengDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Han Kang TeeDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland.
Eirini D TseligkaDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland.
Valeria CagnoDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland.
Gregory MathezDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland.
Stéphane RossetDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland.
Claude M NagamineDepartment of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Peter SarnowDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Karla KirkegaardDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Caroline TapparelDepartment of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland. Electronic address: caroline.tapparel@unige.ch.
University of Geneva · CHStanford University · US

Funding

Viral use and mimicry of autophagy pathway and componentsR01AI134912 · NIAID · STANFORD UNIVERSITY · PI KIRKEGAARD, KARLA · 2018 to 2021
$1.6M
NIAID NIH HHS R01 AI134912
6 · The paper itself

Abstract

Enterovirus A71 (EV-A71) causes hand, foot, and mouth disease outbreaks with neurological complications and deaths. We previously isolated an EV-A71 variant in the stool, cerebrospinal fluid, and blood of an immunocompromised patient who had a leucine-to-arginine substitution on the VP1 capsid protein, resulting in increased heparin sulfate binding. We show here that this mutation increases the virus's pathogenicity in orally infected mice with depleted B cells, which mimics the patient's immune status, and increases susceptibility to neutralizing antibodies. However, a double mutant with even greater heparin sulfate affinity is not pathogenic, suggesting that increased heparin sulfate affinity may trap virions in peripheral tissues and reduce neurovirulence. This research sheds light on the increased pathogenicity of variant with heparin sulfate (HS)-binding ability in individuals with decreased B cell immunity.

Indexed as

EnterovirusEnterovirus A, HumanEnterovirus InfectionsAnimalsAntigens, ViralHeparan SulfateHeparinHumansMiceAntigens, ViralHeparan SulfateHeparinB cellCP: ImmunologyCP: MicrobiologyenterovirusEV71EV-A71heparan sulfatemouseneutralizing antibodyoral infectionpathogenicityVP1

Identifiers

PMID37058406
PMCPMC10590055
OpenAlexW4365445387

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.