Evidence map›Paper›PMID 37056276›Full record

ArticleBioactive materials2023

Upscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile.

Richard Jeske, Chang Liu, Leanne Duke, Maria L Canonicco Castro, Laureana Muok, Peggy Arthur, Mandip Singh, Sunghoon Jung, Li Sun, Yan Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioactive materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed
7.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 86 citations in OpenAlex.

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16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Richard JeskeDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Chang LiuDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Leanne DukeDepartment of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Maria L Canonicco CastroDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Laureana MuokDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Peggy ArthurCollege of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL, USA.
Mandip SinghCollege of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL, USA.
Sunghoon JungPBS Biotech Inc., Camarillo, CA, USA.
Li SunDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Yan LiDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA.
Florida A&M University - Florida State University College of Engineering · USFlorida Agricultural and Mechanical University · USFlorida State University · USProfessional Beef Services · US

Funding

Engineering Extracellular Vesicles of Human Brain Organoids for Stroke TherapyR01NS125016 · NINDS · FLORIDA STATE UNIVERSITY · PI Yan Li · 2022 to 2026
$1.8M
NINDS NIH HHS R01 NS125016
6 · The paper itself

Abstract

Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how bioreactor microenvironment affects the secretion and cargo profiles of hMSC-derived extracellular vesicles (EVs) including the subset, "exosomes", which contain therapeutic proteins, nucleic acids, and lipids from the parent cells. In this study, bone marrow-derived hMSCs were expanded on 3D Synthemax II microcarriers in the PBS mini 0.1L Vertical-Wheel bioreactor system under variable shear stress levels at 25, 40, and 64 RPM (0.1-0.3 dyn/cm

Indexed as

3D microcarriersCargo profileExtracellular vesiclesHuman mesenchymal stromal cellsShear stressVertical-Wheel bioreactor

Identifiers

PMID37056276
PMCPMC10087597
OpenAlexW4289868138

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.