Evidence map›Paper›PMID 37056245›Full record

ArticleBio-protocol2023

Isolation of Immunocomplexes from Zebrafish Brain.

Jennifer Carlisle Michel, Adam C Miller

Open access · diamondAbstract read
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. A zebrafish model ofFrontiers in cell and developmental biology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jennifer Carlisle MichelInstitute of Neuroscience, University of Oregon, Eugene, OR USA.
Adam C MillerInstitute of Neuroscience, University of Oregon, Eugene, OR USA.
University of Oregon · US

Funding

Molecular Mechanisms of Electrical Synapse Formation in VivoR01NS105758 · NINDS · UNIVERSITY OF OREGON · PI Adam C Miller · 2019 to 2026
$3.7M
NINDS NIH HHS R01 NS105758
6 · The paper itself

Abstract

Zebrafish is an excellent model to study vertebrate neurobiology, but its synaptic components that mediate and regulate fast electrical synaptic transmission are largely unidentified. Here, we describe methods to solubilize and immunoprecipitate adult zebrafish brain homogenate under conditions to preserve electrical synapse protein complexes. The methods presented are well-suited to probe electrical synapse immunocomplexes, and potentially other brain-derived immunocomplexes, for candidate interactors from zebrafish brain.

Indexed as

BrainCo-immunoprecipitation (co-IP)Immunoprecipitation (IP)Protein complex purificationWhole tissueZebrafish

Identifiers

PMID37056245
PMCPMC10086547
OpenAlexW4362569414

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.