ArticleNature communications2023
Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 30 citations in OpenAlex.
- Canonical and non-canonical miRNA degradation shapes state transitions and stemness in breast cancer.The EMBO journal · 2026Article
- CLASHub is an integrated database and analytical platform for microRNA-target interactions.Nature communications · 2026Article
- A lncRNA drives developmentally timed decay of all members of an essential microRNA family.Genes & development · 2026Article
- Linking miRNAs to decay.Genes & development · 2026Article
- Article
- Article
- mRNA 3' UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth.Genes & development · 2026Article
- Article
- The E3 ubiquitin ligase mechanism specifying target-directed microRNA degradation.bioRxiv : the preprint server for biology · 2026Article
- Target-Directed miRNA Degradation: Mechanisms and Significance.Methods in molecular biology (Clifton, N.J.) · 2026Article
- mRNA 3' UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth.bioRxiv : the preprint server for biology · 2025Article
- An endogenous cluster of target-directed microRNA degradation sites induces decay of distinct microRNA families.Cell reports · 2025Article
- Regulatory Mechanisms of miRNA Turnover: Insights into ZSWIM8-Mediated Target-Directed MicroRNA Degradation.Biomedicines · 2025Review
- Translation suppresses exogenous target RNA-mediated microRNA decay.Nature communications · 2025Article
- Article
- Widespread destabilization ofRNA (New York, N.Y.) · 2024Article
- An endogenous cluster of target-directed microRNA degradation sites induces decay of distinct microRNA families.bioRxiv : the preprint server for biology · 2024Article
- What goes up must come down: off switches for regulatory RNAs.Genes & development · 2024Review
- Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities.Journal of nanobiotechnology · 2024Review
- To kill a microRNA: emerging concepts in target-directed microRNA degradation.Nucleic acids research · 2024Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNA) load onto AGO proteins to target mRNAs for translational repression or degradation. However, miRNA degradation can be triggered when extensively base-paired with target RNAs, which induces confirmational change of AGO and recruitment of ZSWIM8 ubiquitin ligase to mark AGO for proteasomal degradation. This target RNA-directed miRNA degradation (TDMD) mechanism appears to be evolutionarily conserved, but recent studies have focused on mammalian systems. Here, we performed AGO1-CLASH in Drosophila S2 cells, with Dora (ortholog of vertebrate ZSWIM8) knockout mediated by CRISPR-Cas9 to identify five TDMD triggers (sequences that can induce miRNA degradation). Interestingly, one trigger in the 3' UTR of AGO1 mRNA induces miR-999 degradation. CRISPR-Cas9 knockout of the AGO1 trigger in S2 cells and in Drosophila specifically elevates miR-999, with concurrent repression of the miR-999 targets. AGO1 trigger knockout flies respond poorly to hydrogen peroxide-induced stress, demonstrating the physiological importance of this TDMD event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.