Evidence map›Paper›PMID 37055390›Full record

ArticleNature communications2023

Previously uncharacterized rectangular bacterial structures in the dolphin mouth.

Natasha K Dudek, Jesus G Galaz-Montoya, Handuo Shi, Megan Mayer, Cristina Danita, Arianna I Celis, Tobias Viehboeck, Gong-Her Wu, Barry Behr, Silvia Bulgheresi and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Missing Wedge Completion via Unsupervised Learning with Coordinate Networks.International journal of molecular sciences · 2024
    Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Natasha K DudekDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Jesus G Galaz-MontoyaDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-0113-9325
Handuo ShiDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-1688-6905
Megan MayerDivision of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.
Cristina DanitaDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.
Arianna I CelisDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Tobias ViehboeckDepartment of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria.ORCID 0000-0002-7128-3461
Gong-Her WuDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.
Barry BehrDepartment of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Silvia BulgheresiDepartment of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria.
Kerwyn Casey HuangDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-8043-8138
Wah ChiuDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-8910-3078
David A RelmanDepartment of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA. relman@stanford.edu.ORCID 0000-0001-8331-1354

Funding

3 D ELECTRON MICROSCOPY OF MACROMOLECULES (5P41RR002250-27)P41GM103832 · NIGMS · STANFORD UNIVERSITY · PI CHIU, WAH · 2012 to 2019
$15.1M
A universal pipeline for functional characterization of the human microbiota at a massive scaleRM1GM135102 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BUIE, CULLEN RICHARD, DEUTSCHBAUER, ADAM M · 2020 to 2024
$7.6M
Austrian Science Fund FWF DOC 69NIGMS NIH HHS P41 GM103832NIGMS NIH HHS RM1 GM135102
6 · The paper itself

Abstract

Much remains to be explored regarding the diversity of uncultured, host-associated microbes. Here, we describe rectangular bacterial structures (RBSs) in the mouths of bottlenose dolphins. DNA staining revealed multiple paired bands within RBSs, suggesting the presence of cells dividing along the longitudinal axis. Cryogenic transmission electron microscopy and tomography showed parallel membrane-bound segments that are likely cells, encapsulated by an S-layer-like periodic surface covering. RBSs displayed unusual pilus-like appendages with bundles of threads splayed at the tips. We present multiple lines of evidence, including genomic DNA sequencing of micromanipulated RBSs, 16S rRNA gene sequencing, and fluorescence in situ hybridization, suggesting that RBSs are bacterial and distinct from the genera Simonsiella and Conchiformibius (family Neisseriaceae), with which they share similar morphology and division patterning. Our findings highlight the diversity of novel microbial forms and lifestyles that await characterization using tools complementary to genomics such as microscopy.

Indexed as

Bottle-Nosed DolphinNeisseriaceaeAnimalsBacterial StructuresIn Situ Hybridization, FluorescenceMouthRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID37055390
PMCPMC10102025

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.