ArticleJournal of industrial microbiology & biotechnology2023
Enhanced β-carotene production in Yarrowia lipolytica through the metabolic and fermentation engineering.
Article in Journal of industrial microbiology & biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Integrating Machine Learning and Transcriptomics to Enhance β‑Carotene Production inACS omega · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- CRISPR/Cas9-Mediated Metabolic Engineering of EndophyticACS omega · 2026Article
- Microbial conversion of biodiesel waste for carotenoid production.Frontiers in bioengineering and biotechnology · 2026Article
- Development and application of synthetic biology tools for improved production of human breast milk components.Synthetic and systems biotechnology · 2025Review
- Advances in Lycopene Production: From Natural Sources to Microbial Synthesis UsingMolecules (Basel, Switzerland) · 2025Article
- Combinatorial metabolic engineering of Yarrowia lipolytica for high-level production of the plant-derived diterpenoid sclareol.Microbial cell factories · 2025Article
- Substrate-dependent lipid and β-carotene production in engineered Yarrowia lipolytica: a comparative study.AMB Express · 2025Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
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8 authors at 1 institution in 1 country.
Funding
Abstract
β-Carotene is a kind of high-value tetraterpene compound, which shows various applications in medical, agricultural, and industrial areas owing to its antioxidant, antitumor, and anti-inflammatory activities. In this study, Yarrowia lipolytica was successfully metabolically modified through the construction and optimization of β-carotene biosynthetic pathway for β-carotene production. The β-carotene titer in the engineered strain Yli-C with the introduction of the carotenogenesis genes crtI, crtE, and crtYB can reach 34.5 mg/L. With the overexpression of key gene in the mevalonate pathway and the enhanced expression of the fatty acid synthesis pathway, the β-carotene titer of the engineered strain Yli-CAH reached 87 mg/L, which was 152% higher than that of the strain Yli-C. Through the further expression of the rate-limiting enzyme tHMGR and the copy number of β-carotene synthesis related genes, the β-carotene production of Yli-C2AH2 strain reached 117.5 mg/L. The final strain Yli-C2AH2 produced 2.7 g/L β-carotene titer by fed-batch fermentation in a 5.0-L fermenter. This research will greatly speed up the process of developing microbial cell factories for the commercial production of β-carotene. ONE-SENTENCE SUMMARY: In this study, the β-carotene synthesis pathway in engineered Yarrowia lipolytica was enhanced, and the fermentation conditions were optimized for high β-carotene production.
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