ArticleProceedings of the National Academy of Sciences of the United States of America2023
"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Engineering multiple levels of specificity in an RNA viral vector.Nature communications · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.Nature neuroscience · 2024Article
- Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency.Cell reports methods · 2023Article
- Third-generation rabies viral vectors have low toxicity and improved efficiency as retrograde labeling tools.Cell reports methods · 2023Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA andbioRxiv : the preprint server for biology · 2023Article
- Genomic stability of self-inactivating rabies.eLife · 2023Article
- Hitchhiking on the neuronal highway: Mechanisms of transsynaptic specificity.Journal of chemical neuroanatomy · 2019Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Monosynaptic tracing using rabies virus is an important technique in neuroscience, allowing brain-wide labeling of neurons directly presynaptic to a targeted neuronal population. A 2017 article reported the development of a noncytotoxic version-a major advance-based on attenuating the rabies virus by the addition of a destabilization domain to the C terminus of a viral protein. However, this modification did not appear to hinder the ability of the virus to spread between neurons. We analyzed two viruses provided by the authors and show here that both were mutants that had lost the intended modification, explaining the paper's paradoxical results. We then made a virus that actually did have the intended modification in at least the majority of virions and found that it did not spread efficiently under the conditions described in the original paper, namely, without an exogenous protease being expressed in order to remove the destabilization domain. We found that it did spread when the protease was supplied, although this also appeared to result in the deaths of most source cells by 3 wk postinjection. We conclude that the new approach is not robust but that it could become a viable technique given further optimization and validation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.