ArticlePloS one2023
Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- In vitro effects of green tea (Camellia sinensis) aqueous extract on sperm quality: Mitigating oxidative stress and sperm DNA fragmentation in freeze-dried human spermatozoa.Clinical and experimental reproductive medicine · 2026Article
- Evaluation of dimethylformamide (DMF) and Trehalose as cryoprotectants in African penguin Spheniscus demersus semen cryopreservation.Scientific reports · 2025Article
- Dynamic changes in the sperm quality ofOpen veterinary journal · 2025Article
- Normozoospermic seminal fluid extracellular vesicles could ameliorate adverse effects of freeze-thaw in oligoasthenoteratospermia men.Journal of assisted reproduction and genetics · 2025Article
- A comparative analysis of semen quality traits and sperm kinematic parameters in relation to fertility prediction in Murrah buffaloes.Journal of advanced veterinary and animal research · 2025Article
- Enhanced sperm sexing efficiency and quality preservation in Bali bulls using freeze-dried albumin separation media.Open veterinary journal · 2025Review
- Effects of trehalose supplementation on Pesisir bull sperm quality during storage.Open veterinary journal · 2025Article
- Research progress on the adverse effects of high-altitude environment to the male reproductive system: a review study.Frontiers in endocrinology · 2025Review
- Effect and mechanism of modified Yougui power on Simmental bulls with oligoasthenozoospermia based on targeted amino acid metabolism.Frontiers in veterinary science · 2025Article
- Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples.Antioxidants (Basel, Switzerland) · 2024Article
- A review on the functional roles of trehalose during cryopreservation of small ruminant semen.Frontiers in veterinary science · 2024Review
- The effects of an Aloe-based polyherbal formulation on fertility parameters in male rats.Avicenna journal of phytomedicineArticle
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protection of human sperm during cryopreservation is of great importance to infertility. Recent studies have shown that this area is still a long way from its ultimate aim of maintaining the maximum viability of sperm in cryopreservation. The present study used trehalose and gentiobiose to prepare the human sperm freezing medium during the freezing-thawing. The freezing medium of sperm was prepared with these sugars, and the sperm were then cryopreserved. The viable cells, sperm motility parameters, sperm morphology, membrane integrity, apoptosis, acrosome integrity, DNA fragmentation, mitochondrial membrane potential, reactive oxygen radicals, and malondialdehyde concentration was evaluated using standard protocols. A higher percentage of the total and progressive motility, rate of viable sperm, cell membrane integrity, DNA and acrosome integrity, and mitochondrial membrane potential were observed in the two frozen treatment groups compared to the frozen control. The cells had less abnormal morphology due to treatment with the new freezing medium than the frozen control. The higher malondialdehyde and DNA fragmentation were significantly observed in the two frozen treatment groups than in the frozen control. According to the results of this study, the use of trehalose and gentiobiose in the sperm freezing medium is a suitable strategy for sperm freezing to improve its motion and cellular parameters.
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