ArticleThe Plant cell2023
Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP-L-galactose phosphorylase.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 45 citations in OpenAlex.
- Balancing tuber vitamin C improvement with trade-off side effect by fine-tuning regulation ofHorticulture research · 2026Article
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- Nutritional Supply vs. Flavor Quality: Characterizing the Physicochemical Properties and Amino Acid Profiles of Tomatoes from Beijing and Shandong.Foods (Basel, Switzerland) · 2026Article
- Simultaneous knockout of VITAMIN C DEFECTIVE 2 and 3 exacerbates ascorbate deficiency and light stress sensitivity.Journal of experimental botany · 2025Article
- Manipulation of artificial light environment improves plant biomass and fruit nutritional quality in tomato.Journal of advanced research · 2025Article
- AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit.Horticulture research · 2025Article
- Engineering plant photoreceptors towards enhancing plant productivity.Plant molecular biology · 2025Review
- MAPtools: command-line tools for mapping-by-sequencing and QTL-Seq analysis and visualization.Plant methods · 2024Article
- Continuous Blue Light Treatment Enhances the Nutritional Value of Hydroponically GrownFoods (Basel, Switzerland) · 2024Article
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Deciphering the Genomic Characterization of the GGP Gene Family and Expression Verification ofAntioxidants (Basel, Switzerland) · 2024Article
- Blue and Red LED Lights Differently Affect Growth Responses and Biochemical Parameters in Lentil (Biology · 2023Article
- GmPLP1 negatively regulates soybean resistance to high light stress by modulating photosynthetic capacity and reactive oxygen species accumulation in a blue light-dependent manner.Plant biotechnology journal · 2023Article
- Genome-wide association study unveils ascorbate regulation by PAS/LOV PROTEIN during high light acclimation.Plant physiology · 2023Article
- Out of the blue: blue light mediates ascorbate synthesis.The Plant cell · 2023Article
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22 authors at 3 institutions in 3 countries.
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Abstract
Ascorbate (vitamin C) is an essential antioxidant in fresh fruits and vegetables. To gain insight into the regulation of ascorbate metabolism in plants, we studied mutant tomato plants (Solanum lycopersicum) that produce ascorbate-enriched fruits. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to a knock-out recessive mutation in a class of photoreceptor named PAS/LOV protein (PLP), which acts as a negative regulator of ascorbate biosynthesis. This trait was confirmed by CRISPR/Cas9 gene editing and further found in all plant organs, including fruit that accumulated 2 to 3 times more ascorbate than in the WT. The functional characterization revealed that PLP interacted with the 2 isoforms of GDP-L-galactose phosphorylase (GGP), known as the controlling step of the L-galactose pathway of ascorbate synthesis. The interaction with GGP occurred in the cytoplasm and the nucleus, but was abolished when PLP was truncated. These results were confirmed by a synthetic approach using an animal cell system, which additionally demonstrated that blue light modulated the PLP-GGP interaction. Assays performed in vitro with heterologously expressed GGP and PLP showed that PLP is a noncompetitive inhibitor of GGP that is inactivated after blue light exposure. This discovery provides a greater understanding of the light-dependent regulation of ascorbate metabolism in plants.
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