Evidence map›Paper›PMID 37052702›Full record

ArticleCell and tissue research2023

The long non-coding RNA KLF3-AS1/miR-10a-3p/ZBTB20 axis improves the degenerative changes in human nucleus pulposus cells.

Shijie Chen, Quan Zhuang, Pinghuang Li, Jin Zeng, Yi Peng, Zhiyu Ding, Hongqing Cao, Ruping Zheng, Weiguo Wang

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Cell and tissue research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Shijie Chen *Department of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Quan Zhuang *Transplantation Center, the Third Xiangya Hospital of Central South University, Hunan, 410013, China.
Pinghuang LiDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Jin ZengDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Yi PengDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Zhiyu DingDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Hongqing CaoDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China.
Ruping ZhengSchool of Basic Medical Science, Central South University, Changsha, Hunan, 410013, China.
Weiguo WangDepartment of Orthopaedics, The Third Xiangya Hospital of Central South University, 138 Tongzipo Rd, Changsha, Hunan, 410013, China. csuwwg@csu.edu.cn.ORCID http://orcid.org/0000-0002-3782-9133
Central South University · CN

Funding

National natural science foundation of china 82270795National Natural Science Foundation of China Nos. 81772866National Natural Science Foundation of China Nos. 82172594Natural Science Foundation of Changsha City, China kp2014262Natural Science Foundation of Hunan Province 2021JJ31025
6 · The paper itself

Abstract

Excessive apoptosis of intervertebral disc cells, namely nucleus pulposus (NP) cells, results in decreased cell density and extracellular matrix (ECM) catabolism, hence leading to intervertebral disc degeneration (IVDD). As a cell model in the present study, a commercially available human NP cell line was utilized. Long noncoding RNAs and microRNAs may regulate the proliferation or apoptosis of human NP cells, hence exerting a significant influence on the occurrence of IVDD. KLF3-AS1 was discovered to be abnormally downregulated in IVDD tissues. Overexpression of KLF3-AS1 enhanced NP cell viability, prevented cell apoptosis, boosted ECM synthesis, and lowered MMP-13 and ADAMTS4 levels. ZBTB20 and KLF3-AS1 were co-expressed in IVDD; ZBTB20 overexpression had similar effects on NP cells, ECM production, and MMP-13 and ADAMTS4 levels as KLF3-AS1 overexpression. miR-10a-3p may target KLF3-AS1 and ZBTB20 and inhibit the expression of ZBTB20. Inhibition of miR-10a-3p enhanced NP cell viability, reduced apoptosis, and enhanced ECM synthesis. KLF3-AS1 overexpression increased ZBTB20 expression, whereas miR-10a-3p overexpression decreased ZBTB20 expression; miR-10a-3p overexpression reduced the effects of KLF3-AS1 on ZBTB20. Overexpression of miR-10a-3p consistently decreased the effects of KLF3-AS1 overexpression on NP cell survival, apoptosis, and ECM synthesis. In conclusion, KLF3-AS1 overexpression may ameliorate degenerative NP cell alterations through the miR-10a-3p/ZBTB20 axis.

Indexed as

Intervertebral DiscIntervertebral Disc DegenerationMicroRNAsNucleus PulposusRNA, Long NoncodingApoptosisCell ProliferationHumansKruppel-Like Transcription FactorsMatrix Metalloproteinase 13Nerve Tissue ProteinsTranscription FactorsKLF3 protein, humanKruppel-Like Transcription FactorsMatrix Metalloproteinase 13MicroRNAsMIRN10 microRNA, humanNerve Tissue ProteinsRNA, Long NoncodingTranscription FactorsZBTB20 protein, humanHuman nucleus pulposus (NP) cellsIntervertebral disc degeneration (IVDD)Long non-coding RNA KLF3-AS1miR-10a-3pZBTB20

Identifiers

PMID37052702
OpenAlexW4365444205

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.