Evidence map›Paper›PMID 37051583›Full record

ArticleApplications in plant sciences

Multiple Leaf Sample Extraction System (MuLES): A tool to improve automated morphometric leaf studies.

Christian S Bowman, Ryan Traband, Xuesong Wang, Sara P Knowles, Sassoum Lo, Zhenyu Jia, Nicholi Vorsa, Ira A Herniter

Abstract read
In one paragraph

Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Christian S BowmanDepartment of Botany and Plant Sciences University of California Riverside, 2142 Batchelor Hall Riverside California 92521 USA.ORCID 0000-0002-5199-985X
Ryan TrabandDepartment of Botany and Plant Sciences University of California Riverside, 2142 Batchelor Hall Riverside California 92521 USA.ORCID 0000-0003-2340-6348
Xuesong WangDepartment of Botany and Plant Sciences University of California Riverside, 2142 Batchelor Hall Riverside California 92521 USA.
Sara P KnowlesDepartment of Plant Biology Rutgers University 59 Dudley Road New Brunswick New Jersey 08901 USA.
Sassoum LoDepartment of Plant Sciences University of California, Davis, One Shields Avenue Davis California 95616 USA.ORCID 0000-0002-0385-5691
Zhenyu JiaDepartment of Botany and Plant Sciences University of California Riverside, 2142 Batchelor Hall Riverside California 92521 USA.ORCID 0000-0002-1428-904X
Nicholi VorsaDepartment of Plant Biology Rutgers University 59 Dudley Road New Brunswick New Jersey 08901 USA.ORCID 0000-0002-7976-3486
Ira A HerniterDepartment of Plant Biology Rutgers University 59 Dudley Road New Brunswick New Jersey 08901 USA.ORCID 0000-0001-5662-083X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Premise: The measurement of leaf morphometric parameters from digital images can be time-consuming or restrictive when using digital image analysis softwares. The Multiple Leaf Sample Extraction System (MuLES) is a new tool that enables high-throughput leaf shape analysis with minimal user input or prerequisites, such as coding knowledge or image modification. Methods and Results: MuLES uses contrasting pixel color values to distinguish between leaf objects and their background area, eliminating the need for color threshold-based methods or color correction cards typically required in other software methods. The leaf morphometric parameters measured by this software, especially leaf aspect ratio, were able to distinguish between large populations of different accessions for the same species in a high-throughput manner. Conclusions: MuLES provides a simple method for the rapid measurement of leaf morphometric parameters in large plant populations from digital images and demonstrates the ability of leaf aspect ratio to distinguish between closely related plant types.

Indexed as

aspect ratiobreadthFeret diameterleaf shapemorphometricsshape analysis

Identifiers

PMID37051583
PMCPMC10083438

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.