Development of a Target-to-Sensor Mode Multispectral Imaging Device for High-Throughput and High-Precision Touch-Based Leaf-Scale Soybean Phenotyping.

Image-based spectroscopy phenotyping is a rapidly growing field that investigates how genotype, environment and management interact using remote or proximal sensing systems to capture images of a plant under multiple wavelengths of light. While remote sensing techniques have proven effective in crop phenotyping, they can be subject to various noise sources, such as varying lighting conditions and plant physiological status, including leaf orientation. Moreover, current proximal leaf-scale imaging devices require the sensors to accommodate the state of the samples during imaging which induced extra time and labor cost. Therefore, this study developed a proximal multispectral imaging device that can actively attract the leaf to the sensing area (target-to-sensor mode) for high-precision and high-throughput leaf-scale phenotyping. To increase the throughput and to optimize imaging results, this device innovatively uses active airflow to reposition and flatten the soybean leaf. This novel mechanism redefines the traditional sensor-to-target mode and has relieved the device operator from the labor of capturing and holding the leaf, resulting in a five-fold increase in imaging speed compared to conventional proximal whole leaf imaging device. Besides, this device uses artificial lights to create stable and consistent lighting conditions to further improve the quality of the images. Furthermore, the touch-based imaging device takes full advantage of proximal sensing by providing ultra-high spatial resolution and quality of each pixel by blocking the noises induced by ambient lighting variances. The images captured by this device have been tested in the field and proven effective. Specifically, it has successfully identified nitrogen deficiency treatment at an earlier stage than a typical remote sensing system. The