ArticlePlants (Basel, Switzerland)2023
Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus.
Article in Plants (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Asymmetric LAMP-gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts.RSC advances · 2026Article
- Development of a Rapid and Sensitive RT-qPCR for the Specific Detection of Citrus Viroid V and Its Field Application.Viruses · 2026Article
- Article
- Rapid LAMP-Based Detection of Mixed Begomovirus Infections in Field-Grown Tomato Plants.Viruses · 2025Article
- Emerging technologies for in-field plant virus detection: innovations and future directions.The Journal of general virology · 2025Review
- Development and validation of LAMP and real-time LAMP assays for rapid detection of coccinia mosaic Virudhunagar virus in bitter gourd (3 Biotech · 2025Article
- Article
- Identification of Pathogen Causing Bulb Rot inPlants (Basel, Switzerland) · 2024Article
- Recent advances and challenges in plant viral diagnostics.Frontiers in plant science · 2024Review
- Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteriaJournal of Indian Society of PeriodontologyArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions on zucchini squash, tomato, and pepper samples. A set of six LAMP primers was designed for specific ToCLNDV detection, targeting a 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity and accuracy of the real-time LAMP assay and comparison with canonical PCR were evaluated. The real-time LAMP assay developed was about one-thousand times more sensitive than the conventional PCR method, detecting a total of 4.41 × 10
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Registered trials
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