ReviewInternational journal of molecular sciences2023
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.
Review in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- 3D radiomics profiling of thyroid tumors using micro-CT.Scientific reports · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction.Microscopy research and technique · 2025Article
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.Communications biology · 2025Article
- Tellurium-Doped Silanised Bioactive Glass-Chitosan Hydrogels: A Dual Action for Antimicrobial and Osteoconductive Platforms.Polymers · 2025Article
- Three-dimensional imaging and computational quantitation as a novel approach to assess nerve fibers, enteric glial cells, mast cells, and the proximity of mast cells to the nerve fibers in human sigmoid mucosal biopsies from healthy subjects.Journal of neuroscience methods · 2025Article
- 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.Redox biology · 2025Article
- Towards MRI Study of Biointegration of Carbon-Carbon Composites with Ca-P Coatings.Nanomaterials (Basel, Switzerland) · 2025Article
- Brain-wide immunolabeling and tissue clearing applications for engram research.Neurobiology of learning and memory · 2025Review
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- Review
- The potential of x-ray virtual histology in the diagnosis of skin tumors.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Novel strategy to preserve valve morphology for 3-dimensional analysis of rat pulmonary valves.JTCVS structural and endovascular · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- Engineering the future of 3D pathology.The journal of pathology. Clinical research · 2024Article
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence nuclear and eosin Y staining that enabled us to obtain hematoxylin and eosin pseudo-coloring comparable with the gold standard H&E analysis. The computational reconstructions obtained with 3D optical imaging can be analyzed by a pathologist without any specific training in volumetric microscopy, paving the way for new biomedical applications in clinical pathology.
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Registered trials
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