Multiplexed Fluorescence Plate Reader In Situ Protein Expression Assay in Apoptotic HepG2 Cells.

Rita Jakabfi-Csepregi, Gábor L Kovács, Péter Kaltenecker, Tamás Kőszegi

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.6field-weighted citation impact, top 31% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Inhibition of Foxp3 expression in the placenta of mice infected intraperitoneally by toxoplasma gondii tachyzoites: insights into the PPARγ/miR-7b-5p/Sp1 signaling pathway.Parasites & vectors · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Rita Jakabfi-CsepregiDepartment of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.

Gábor L KovácsDepartment of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.ORCID 0000-0001-5298-5401

Péter KalteneckerSzentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.ORCID 0000-0002-0151-4682

Tamás KőszegiDepartment of Laboratory Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.ORCID 0000-0002-3910-5745

University of Pecs · HU

Funding

National Laboratory on Human Reproduction RRF-2.3.1-21-2022-00012University of Pécs Thematic Excellence Program 2020 - National Excellence Sub-program of the Ministry for Innova-tion and Technology in Hungary

6 · The paper itself

Abstract

Instead of Western blot being considered as a gold standard for intracellular protein expression assays, we developed a novel multiplexed high throughput (180 tests/day) in situ manual protein expression method directly in 96-well plates using 25,000-100,000 cells/well after formaldehyde fixation and Triton X 100 permeabilization. HepG2 cells were treated with ochratoxin A (OTA) and staurosporine (STP) to induce apoptosis. Antioxidant and apoptotic cell signaling protein expression were studied by various rabbit primary antibodies and HRP labeled secondary antibodies. The HRP labeled immune complexes were developed by H

Indexed as

NF-kappa BProto-Oncogene Proteins c-aktAMP-Activated Protein KinasesAnimalsApoptosisbcl-2-Associated X ProteinCaspase 3FluorescenceHep G2 CellsHumansHydrogen Peroxidep38 Mitogen-Activated Protein KinasesPPAR gammaRabbitsAMP-Activated Protein Kinasesbcl-2-Associated X ProteinCaspase 3Hydrogen PeroxideNF-kappa Bp38 Mitogen-Activated Protein KinasesPPAR gammaProto-Oncogene Proteins c-aktAmpliflu Redapoptosisdose–response analysisfluorescenceHepG2 cellsmultiplexed assayplate readersignal protein expression

Identifiers

PMID37047536

DOI10.3390/ijms24076564

PMCPMC10094837

OpenAlexW4362588500

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.