Evidence map›Paper›PMID 37047388›Full record

ReviewInternational journal of molecular sciences2023

Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers.

Juya Jeon, Shri Venkatesh Subramani, Kok Zhi Lee, Bojing Jiang, Fuzhong Zhang

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026
    Review
  2. Article
  3. Review
  4. Article
  5. EnhancingApplied and environmental microbiology · 2025
    Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Juya JeonDepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.ORCID 0000-0001-7723-9413
Shri Venkatesh SubramaniDepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.ORCID 0009-0000-4101-2858
Kok Zhi LeeDepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.ORCID 0000-0002-1836-2662
Bojing JiangDepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.ORCID 0000-0001-6674-0464
Fuzhong ZhangDepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.ORCID 0000-0001-6979-7909

Funding

Targeted delivery of a proangiogenic and promyogenic protein for regeneration of diabetic ischemic limbsR01HL164062 · NHLBI · WASHINGTON UNIVERSITY · PI GUAN, JIANJUN, ZHANG, FUZHONG · 2022 to 2025
$2.3M
NIH HHS R01HL164062
6 · The paper itself

Abstract

High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications.

Indexed as

PolymersProteinsMolecular WeightRepetitive Sequences, Nucleic AcidPolymersProteinshigh molecular weightmicrobial synthesisprotein polymersrepetitive proteinssilksynthetic biology

Identifiers

PMID37047388
PMCPMC10094428

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.