Evidence map›Paper›PMID 37046148›Full record

ArticleArchives of virology2023

Seed transmission of raspberry bushy dwarf virus is blocked in Nicotiana benthamiana plants by preventing virus entry into the embryo from the infected embryo sac and endosperm.

Masamichi Isogai, Mizuna Yoshikoshi, Kentaro Seki, Hiromi Masuko-Suzuki, Masao Watanabe, Kouki Matsuo, Hajime Yaegashi

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Masamichi IsogaiPlant Pathology Laboratory, Faculty of Agriculture, Iwate University, 18-8, Ueda 3-chome, Morioka, 020-8550, Japan. isogai@iwate-u.ac.jp.ORCID http://orcid.org/0000-0002-3095-6439
Mizuna YoshikoshiPlant Pathology Laboratory, Faculty of Agriculture, Iwate University, 18-8, Ueda 3-chome, Morioka, 020-8550, Japan.
Kentaro SekiPlant Pathology Laboratory, Faculty of Agriculture, Iwate University, 18-8, Ueda 3-chome, Morioka, 020-8550, Japan.
Hiromi Masuko-SuzukiGraduate School of Life Sciences, Tohoku University, 1-1, Katahira 2-chome, Aoba-ku, Sendai, 980-8577, Japan.
Masao WatanabeGraduate School of Life Sciences, Tohoku University, 1-1, Katahira 2-chome, Aoba-ku, Sendai, 980-8577, Japan.
Kouki MatsuoBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517, Japan.
Hajime YaegashiPlant Pathology Laboratory, Faculty of Agriculture, Iwate University, 18-8, Ueda 3-chome, Morioka, 020-8550, Japan.

Funding

Japan Society for the Promotion of Science 21K05590
6 · The paper itself

Abstract

Raspberry bushy dwarf virus (RBDV) is transmitted through seed in infected red raspberry plants after pollination with pollen grains from healthy red raspberry plants. Here, we show that RBDV is not transmitted through seeds in infected Nicotiana benthamiana (Nb) plants after pollination with virus-free Nb pollen grains. Chromogenic in situ hybridization revealed that the virus invades the shoot apical meristem and the ovule, including the embryo sac, of RBDV-infected Nb plants; however, in seeds that developed from infected embryo sacs after fertilization by virus-free sperm cells, RBDV was absent in the embryos and present in the endosperms. When we analyzed seed transmission of RBDV in Nb mutants with mutations in dicer-like enzyme 2 and 4 (NbDCL2&4) or RNA-dependent RNA polymerase 6 (NbRDR6), RBDV was not present in the offspring from seeds with embryos and endosperms that did not express NbDCL2&4 or NbRDR6. These results suggest that seed transmission of RBDV is prevented by evasion of infection by the embryo and that RNA silencing is not essential for preventing seed transmission of RBDV in Nb plants.

Indexed as

Plant VirusesRNA VirusesRubusEndospermNicotianaPlant DiseasesSeedsVirus Internalization

Identifiers

PMID37046148

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.