Evidence map›Paper›PMID 37046118›Full record

ArticleMikrochimica acta2023

Upconversion nanoparticle-based fluorescence resonance energy transfer sensing platform for the detection of cathepsin B activity in vitro and in vivo.

Xinxin Li, Meiling Zhang, Hua Zhang, Zhenxin Wang, Huimao Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Xinxin Li *Department of Radiology, The First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
Meiling Zhang *Department of Radiology, The First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
Hua ZhangState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People's Republic of China. zhanghua@ciac.ac.cn.
Zhenxin WangState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People's Republic of China.
Huimao ZhangDepartment of Radiology, The First Hospital of Jilin University, Changchun, 130021, People's Republic of China. huimao@jlu.edu.cn.
First Hospital of Jilin University · CNChangchun Institute of Applied Chemistry · CNChinese Academy of Sciences · CNJilin University · CN

Funding

Achievement Transformation Guidance Fund Between The First Hospital of Jilin University and Changchun Institute of Applied Chemistry CGZHYD202012-009National Natural Science Foundation of China 61901438National Natural Science Foundation of China 81871406
6 · The paper itself

Abstract

A simple fluorescence resonance energy transfer (FRET) sensing platform (termed as USP), comprised of upconversion nanoparticles (UCNPs) as the energy donor and Cy5 as the energy acceptor, has been synthesized for cathepsin B (CTSB) activity detection in vitro and in vivo. When Cy5-modified peptide substrate (peptide-Cy5) of CTSB is covalently linked on the surface of UCNPs, the FRET between the UCNPs (excitation: 980 nm; emission: 541 nm/655 nm) and Cy5 (excitation: 645 nm) leads to a reduction in the red upconversion luminescence (UCL) signal intensity of UCNPs. Cy5 can be liberated from UCNPs in the presence of CTSB through the cleavage of peptide-Cy5 by CTSB, leading to the recovery of the red UCL signal of UCNPs. Because the green UCL signal of UCNPs remains constant during the CTSB digestion, it can be considered as an internal reference. The findings demonstrate the ability of USP to detect CTSB with the linear detection ranges of 1 to 100 ng·mL

Indexed as

Fluorescence Resonance Energy TransferNanoparticlesAnimalsCarbocyaninesCathepsin BMicePeptidesCarbocyaninesCathepsin BCtsb protein, mousecyanine dye 5PeptidesCathepsin B activityColorectal cancerFluorescence resonance energy transferUpconversion luminescence imaging

Identifiers

PMID37046118
OpenAlexW4365137101

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.