ArticleScientific reports2023
Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- Exploring the Role of E. Coli Nissle 1917 Postbiotics as Antimicrobial and Antioxidant Agents for Enhancing Buffalo Sperm Quality.Probiotics and antimicrobial proteins · 2026Article
- Addition of lactic acid bacteria to diluted ram semen as vehicle for vaginal inoculation: interaction with seminal microbiota, sperm quality and antibacterial in vitro effect against Mycoplasma agalactiae.BMC veterinary research · 2026Article
- Weissella: A multifaceted genus making the way for next-gen biotherapeutics?Antonie van Leeuwenhoek · 2026Review
- Postbiotic approach to antibiotic resistance: antibacterial and antibiofilm potential of Lactobacillus salivarius supernatant.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Use of a synthetic antimicrobial peptide (PA-13) alone or in combination with reduced gentamicin levels to controlVeterinary world · 2026Article
- From Production to Application: Postbiotics in Meat, Meat Products, Other Food Matrices, and Bioactive Packaging.Foods (Basel, Switzerland) · 2026Review
- Cell-Free but potent: Antimicrobial potential of Lactobacillus supernatants against multidrug-resistant pathogens.PloS one · 2026Article
- Bactericidal, Anti-Biofilm, and Stress-Response Modulatory Effects ofFoods (Basel, Switzerland) · 2025Article
- Article
- Genomic and Biological Characterization of a NovelViruses · 2025Article
- Human gut microbiota-derived antimicrobials against Salmonella Typhi: diversity, mechanisms, and therapeutic potential.Archives of microbiology · 2025Review
- Article
- Inhibitory effect of cell-free supernatants ofVeterinary research forum : an international quarterly journal · 2025Article
- First detection of multidrug-resistant and toxigenic Pasteurella aerogenes in sow vaginal discharge: a novel threat to swine health in Thailand.Scientific reports · 2024Article
- Genomic Insights intoAntibiotics (Basel, Switzerland) · 2024Article
- Biogenic amine tryptamine in human vaginal probiotic isolates mediates matrix inhibition and thwarts uropathogenic E. coli biofilm.Scientific reports · 2024Article
- Effect of Antimicrobial Peptide BiF2_5K7K on Contaminated Bacteria Isolated from Boar Semen and Semen Qualities during Preservation and Subsequent Fertility Test on Pig Farm.Antibiotics (Basel, Switzerland) · 2024Article
- The Effects of Different Antimicrobial Peptides (A-11 and AP19) on Isolated Bacteria from Fresh Boar Semen and Semen Quality during Storage at 18 °C.Antibiotics (Basel, Switzerland) · 2024Article
- In-vitro selection of lactic acid bacteria to combat Salmonella enterica and Campylobacter jejuni in broiler chickens.World journal of microbiology & biotechnology · 2024Article
- Antioxidant and Antibacterial Activities of Nano-probiotics Versus Free Probiotics Against Gastrointestinal Pathogenic Bacteria.Indian journal of microbiology · 2024Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of antibiotics with semen extender appears to be a practical solution to minimise bacterial growth in fresh boar semen preservation. Unfortunately, the excessive use of antibiotics promotes antimicrobial resistance (AMR). This becomes a worldwide concern due to the antimicrobial resistance genes transmitted to animals, environment, and humans. Probiotics are one of the alternative methods to reduce antibiotic use. They could inhibit pathogenic bacteria by producing antimicrobial substances in cell free supernatants (CFS). Nevertheless, there is no comprehensive study undertaken on inhibitory activity against pathogenic bacteria isolated from boar semen origin. Our study investigated the efficacy of CFS produced from selected probiotics: Bacillus spp., Enterococcus spp., Weissella spp., Lactobacillus spp., and Pediococcus spp. inhibiting pathogenic bacteria isolated from fresh boar semen. Besides, the semen-origin pathogenic bacteria are subjected to identification, antimicrobial resistance genes detection, and antibiotic susceptibility test (AST). Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis are the most common pathogens identified in boar semen with resistance to numerous antibiotics used in pig industry. The CFS with its antimicrobial peptides and/or bacteriocin constituent derived from selected probiotics could inhibit the growth of pathogenic bacteria carrying antimicrobial resistance genes (mcr-3 and int1 genes). The inhibition zones for Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis provided more efficient results in the CFS derived from Lactobacillus spp. and Pediococcus spp. than those of the CFS produced from Enterococcus spp., Weissella spp. and Bacillus spp., respectively. It is worth noted that as the incubation time increased, the antibacterial activity decreased conversely. Our results on CFS with its antimicrobial peptides and/or bacteriocin constituent inhibits semen-origin pathogenic bacteria guide the direction as a promising alternative method used in the semen extender preservation of the pig industry.
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