ArticleGenes and environment : the official journal of the Japanese Environmental Mutagen Society2023
In vivo mutagenicity assessment of styrene in MutaMouse liver and lung.
Article in Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Increased mutagenicity in the liver of MutaMouse females following oral treatment with commercial-grade toluene diisocyanate.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2025Article
- Re-assessment of the risks to public health related to the genotoxicity of styrene present in plastic food contact materials.EFSA journal. European Food Safety Authority · 2025Article
- Determination of 4-tert-butyl pyrocatechol content in styrene with high accuracy using a chromatographic device under safe conditions: a laboratory study.Scientific reports · 2025Article
- Lack of in vivo mutagenicity of carbendazim in the liver and glandular stomach of MutaMice.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2024Article
- Investigation of mutagenicity of styrene in tumor target and non-target tissues of transgenic Big Blue® mice.Environmental and molecular mutagenesisArticle
Corrections and comments
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Authors and funding
11 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStyrene (CAS 100-42-5) is widely used as polystyrene and acrylonitrile-butadiene-styrene resin such as plastic, rubber, and paint. One of the primary uses of styrene is food utensils and containers, but a small amount of styrene transferred into food can be ingested by eating. Styrene is metabolized into styrene 7,8-oxide (SO). SO is mutagenic in bacteria and mouse lymphoma assays. It is clastogenic in cultured mammalian cells. However, styrene and SO are not clastogenic/aneugenic in rodents, and no rodent in vivo gene mutation studies were identified.
methodsTo investigate the mutagenicity of orally administered styrene, we used the transgenic rodent gene mutation assay to perform an in vivo mutagenicity test (OECD TG488). The transgenic MutaMouse was given styrene orally at doses of 0 (corn oil; negative control), 75, 150, and 300 mg/kg/day for 28 days, and mutant frequencies (MFs) were determined using the lacZ assay in the liver and lung (five male mice/group).
resultsThere were no significant differences in the MFs of the liver and lung up to 300 mg/kg/day (close to maximum tolerable dose (MTD)), when one animal with extremely high MFs that were attributed to an incidental clonal mutation was omitted. Positive and negative controls produced the expected results.
conclusionsThese findings show that styrene is not mutagenic in the liver and lung of MutaMouse under this experimental condition.
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