ArticleMethods in molecular biology (Clifton, N.J.)2023
Production of Cloned Pigs by Handmade Cloning.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- Large animal models in gynecology: status and future perspectives.Frontiers in veterinary science · 2025Review
- Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos.Journal of animal science and technology · 2024Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
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Abstract
Somatic cell nuclear transfer (SCNT) in pigs is a promising technology in biomedical research by association with transgenesis for xenotransplantation and disease modeling technologies. Handmade cloning (HMC) is a simplified SCNT method that does not require micromanipulators and facilitates the generation of cloned embryos in large quantities. As a result of HMC fine-tuning for porcine-specific requirements of both oocytes and embryos, HMC has become uniquely efficient (>40% blastocyst rate, 80-90% pregnancy rates, 6-7 healthy offspring per farrowing, and with negligible losses and malformations). Therefore, this chapter describes our HMC protocol to obtain cloned pigs.
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