Evidence map›Paper›PMID 37041280›Full record

ArticleCommunications biology2023

Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells using non-integrating vectors.

Wanqiu Chen, Chenguang Wang, Zhi-Xue Yang, Feng Zhang, Wei Wen, Christoph Schaniel, Xianqiang Mi, Matthew Bock, Xiao-Bing Zhang, Hongyu Qiu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Wanqiu ChenCenter for Genomics, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
Chenguang WangCenter for Genomics, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
Zhi-Xue YangDepartment of Medicine, Loma Linda University, Loma Linda, CA, USA.
Feng ZhangDepartment of Medicine, Loma Linda University, Loma Linda, CA, USA.
Wei WenDepartment of Medicine, Loma Linda University, Loma Linda, CA, USA.
Christoph SchanielDivision of Hematology and Medical Oncology, Black Family Stem Cell Institute, Tisch Cancer Institute, Mount Sinai Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID 0000-0002-1343-4858
Xianqiang MiShanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences, Shanghai, China.
Matthew BockDepartment of Pediatrics, School of Medicine, Loma Linda University, Loma Linda, CA, USA.ORCID 0000-0003-1357-4698
Xiao-Bing ZhangDepartment of Medicine, Loma Linda University, Loma Linda, CA, USA. zhangxbhk@gmail.com.ORCID 0000-0003-4319-6181
Hongyu QiuTranslational Cardiovascular Research Center, Department of Internal Medicine, University of Arizona - College of Medicine at Phoenix, Phoenix, AZ, USA. hqiu@arizona.edu.ORCID 0000-0002-2436-8921
Charles WangCenter for Genomics, School of Medicine, Loma Linda University, Loma Linda, CA, USA. chwang@llu.edu.ORCID 0000-0001-8861-2121
Loma Linda University · USIcahn School of Medicine at Mount Sinai · USShanghai Institute of Microsystem and Information Technology · CNUniversity of Arizona · US

Funding

Intrinsic Stiffness of Aortic Vascular Smooth Muscle Cell in the Development of HypertensionR01HL115195 · NHLBI · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI QIU, HONGYU · 2013 to 2022
$5.1M
Role of VCP in coronary ischemic injuryR01HL142291 · NHLBI · LOMA LINDA UNIVERSITY · PI QIU, HONGYU, XIE, ZHONGLIN · 2018 to 2021
$2.6M
Novel mechanism mediating cardiac protection upon pressure overloadR01HL137962 · NHLBI · LOMA LINDA UNIVERSITY · PI QIU, HONGYU · 2018 to 2020
$1.2M
Novel mechanisms mediating cardiac protection upon pressure overloadR56HL137962 · NHLBI · LOMA LINDA UNIVERSITY · PI QIU, HONGYU · 2017 to 2017
$393k
High Throughput DNA SequencerS10OD019960 · OD · LOMA LINDA UNIVERSITY · PI WANG, CHARLES · 2015 to 2015
$272k
NHLBI NIH HHS R01 HL115195NHLBI NIH HHS R01 HL137962NHLBI NIH HHS R01 HL142291NHLBI NIH HHS R56 HL137962NIH HHS S10 OD019960
6 · The paper itself

Abstract

Mesenchymal stromal cells (MSCs) have great value in cell therapies. The MSC therapies have many challenges due to its inconsistent potency and limited quantity. Here, we report a strategy to generate induced MSCs (iMSCs) by directly reprogramming human peripheral blood mononuclear cells (PBMCs) with OCT4, SOX9, MYC, KLF4, and BCL-XL using a nonintegrating episomal vector system. While OCT4 was not required to reprogram PBMCs into iMSCs, omission of OCT4 significantly impaired iMSC functionality. The omission of OCT4 resulted in significantly downregulating MSC lineage specific and mesoderm-regulating genes, including SRPX, COL5A1, SOX4, SALL4, TWIST1. When reprogramming PBMCs in the absence of OCT4, 67 genes were significantly hypermethylated with reduced transcriptional expression. These data indicate that transient expression of OCT4 may serve as a universal reprogramming factor by increasing chromatin accessibility and promoting demethylation. Our findings represent an approach to produce functional MSCs, and aid in identifying putative function associated MSC markers.

Indexed as

Leukocytes, MononuclearMesenchymal Stem CellsCell DifferentiationHumansOctamer Transcription Factor-3PlasmidsSOXC Transcription FactorsOctamer Transcription Factor-3SOX4 protein, humanSOXC Transcription Factors

Identifiers

PMID37041280
PMCPMC10090171
OpenAlexW4364366694

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.