Evidence map›Paper›PMID 37040415›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

Gas-modulating microcapsules for spatiotemporal control of hypoxia.

Thomas G Molley, Shouyuan Jiang, Louis Ong, Chantal Kopecky, Chavinya D Ranaweera, Gagan K Jalandhra, Laura Milton, Egi Kardia, Zeheng Zhou, Jelena Rnjak-Kovacina and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026
    Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Thomas G MolleySchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Shouyuan JiangGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Louis OngSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD 4000, Australia.
Chantal KopeckySchool of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
Chavinya D RanaweeraSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
Gagan K JalandhraSchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Laura MiltonSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD 4000, Australia.
Egi KardiaSchool of Biomedical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Zeheng ZhouSchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Jelena Rnjak-KovacinaGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Shafagh A WatersSchool of Biomedical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0003-1144-5411
Yi-Chin TohSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD 4000, Australia.
Kristopher A KilianSchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0002-8963-9796
UNSW Sydney · AUQueensland University of Technology · AU

Funding

Engineered microtumor arrays for development of combination therapiesR01CA251443 · NCI · UNIVERSITY OF NEW SOUTH WALES · PI COPLAND, JOHN A., KILIAN, KRISTOPHER ALAN · 2020 to 2024
$998k
NCI NIH HHS R01 CA251443
6 · The paper itself

Abstract

Oxygen is a vital molecule involved in regulating development, homeostasis, and disease. The oxygen levels in tissue vary from 1 to 14% with deviations from homeostasis impacting regulation of various physiological processes. In this work, we developed an approach to encapsulate enzymes at high loading capacity, which precisely controls the oxygen content in cell culture. Here, a single microcapsule is able to locally perturb the oxygen balance, and varying the concentration and distribution of matrix-embedded microcapsules provides spatiotemporal control. We demonstrate attenuation of hypoxia signaling in populations of stem cells, cancer cells, endothelial cells, cancer spheroids, and intestinal organoids. Varying capsule placement, media formulation, and timing of replenishment yields tunable oxygen gradients, with concurrent spatial growth and morphogenesis in a single well. Capsule containing hydrogel films applied to chick chorioallantoic membranes encourages neovascularization, providing scope for topical treatments or hydrogel wound dressings. This platform can be used in a variety of formats, including deposition in hydrogels, as granular solids for 3D bioprinting, and as injectable biomaterials. Overall, this platform's simplicity and flexibility will prove useful for fundamental studies of oxygen-mediated processes in virtually any in vitro or in vivo format, with scope for inclusion in biomedical materials for treating injury or disease.

Indexed as

Endothelial CellsHypoxiaBiocompatible MaterialsCapsulesHumansHydrogelsOxygenBiocompatible MaterialsCapsulesHydrogelsOxygenbiomaterialscancerhypoxiamicrocapsulesstem cells

Identifiers

PMID37040415
PMCPMC10120079
OpenAlexW4364354369

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.