Evidence map›Paper›PMID 37039994›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Methods for Culturing Anaerobic Microorganisms.

Michel Geovanni Santiago-Martínez, James Gregory Ferry

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
5.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Aerotolerant capacity of the lung symbiontJournal of bacteriology · 2026
    Article
  2. Article
  3. Article
  4. Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024
    Review
  5. In vivo structure probing of RNA inRNA (New York, N.Y.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Michel Geovanni Santiago-MartínezDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA. geo_santiagom@uconn.edu.
James Gregory FerryDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA.
Pennsylvania State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anaerobic microorganisms (anaerobes) proliferate in diverse oxygen-free environments. They inhabit Earth's soils and aquatic sediments, the rumen and gut of mammals, and the gut of insects among many other oxygen-free environments. Anaerobes impact biotechnological, biomedical, ecological, and astrobiological fields. Sensitivity to oxygen is of prime consideration for successful culturing which is essential to understand function. Although cultivated for many years, the protocols and media components have been modified and adapted to the special needs of species, as well as conditions and variables for experimental evaluations. Here we describe a revised method used in our laboratories for the growth of methane-producing anaerobes (methanogenic archaea) which are among the most oxygen sensitive. The method is an example for the preparation of more specific media to cultivate a wide diversity of anaerobes.

Indexed as

ArchaeaBacteria, AnaerobicAnaerobiosisBiotechnologyMethaneMethaneAnaerobesAnaerobic mediaArchaeaBacteriaOxygenRedox indicatorResazurin

Identifiers

PMID37039994
OpenAlexW4364351711

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.