Evidence map›Paper›PMID 37039644›Full record

ArticleACS synthetic biology2023

Investigating and Optimizing the Lysate-Based Expression of Nonribosomal Peptide Synthetases Using a Reporter System.

Jaime Lorenzo N Dinglasan, Tien T Sword, J William Barker, Mitchel J Doktycz, Constance B Bailey

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jaime Lorenzo N DinglasanGraduate School of Genome Science & Technology, University of Tennessee-Knoxville, Knoxville, Tennessee 37996, United States.
Tien T SwordDepartment of Chemistry, University of Tennessee-Knoxville, Knoxville, Tennessee 37996, United States.
J William BarkerDepartment of Chemistry, University of Tennessee-Knoxville, Knoxville, Tennessee 37996, United States.
Mitchel J DoktyczBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.ORCID 0000-0003-4856-8343
Constance B BaileyDepartment of Chemistry, University of Tennessee-Knoxville, Knoxville, Tennessee 37996, United States.ORCID 0000-0002-4668-7245
University of Tennessee at Knoxville · USOak Ridge National Laboratory · US

Funding

Mechanistic Studies of the Functional Consequences of Heterologous Expression of Actinomycetal MegasynthasesR15GM146192 · NIGMS · UNIVERSITY OF TENNESSEE KNOXVILLE · PI BACCILE, JOSHUA A · 2022 to 2022
$432k
NIGMS NIH HHS R15 GM146192
6 · The paper itself

Abstract

Lysate-based cell-free expression (CFE) systems are accessible platforms for expressing proteins that are difficult to synthesize

Indexed as

Escherichia coliPeptide SynthasesPeptidesnon-ribosomal peptide synthasePeptidesPeptide Synthasesblue pigment synthetase Acell-free protein expressionfluorescent labelingnatural productsnonribosomal peptide synthetase

Identifiers

PMID37039644
PMCPMC11236431
OpenAlexW4364352492

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.