ArticleJournal of biomedical optics2023
Minimizing near-infrared autofluorescence in preclinical imaging with diet and wavelength selection.
Article in Journal of biomedical optics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
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- Combining Fluorescence and Magnetic Resonance Imaging in Drug Discovery-A Review.Pharmaceuticals (Basel, Switzerland) · 2025Review
- High dynamic range shortwave infrared (SWIR) imaging of mice with an InGaAs camera.bioRxiv : the preprint server for biology · 2025Article
- Making Fluorescent Nylon, Polypropylene, and Polystyrene Microplastics for In Vivo and In Vitro Imaging.Microplastics (Basel, Switzerland) · 2025Article
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- Advances in Optical Contrast Agents for Medical Imaging: Fluorescent Probes and Molecular Imaging.Journal of imaging · 2025Review
- Systematic comparison of fluorescence imaging in the near-infrared and shortwave-infrared spectral range using clinical tumor samples containing cetuximab-IRDye800CW.Journal of biomedical optics · 2025Article
- Multiplexed Shortwave Infrared Imaging Highlights Anatomical Structures in Mice.Angewandte Chemie (International ed. in English) · 2024Article
- Depth detection limit of a fluorescent object in tissue-like medium with background emission in continuous-wave measurements: a phantom study.Journal of biomedical optics · 2024Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Significance: Preclinical fluorescence imaging with NIR-I (700 to 900 nm) illumination and short-wave infrared or NIR-II (1000 to 1700 nm) emission increases tissue penetration depth and improves resolution through decreased scattering. Background autofluorescence decreases signal-to-background ratios (SBR) in fluorescence imaging; maximizing SBR will further improve the impact of deep tissue imaging. Aim: The impact of rodent diet, illumination wavelength, and emission range on the background fluorescence and contrast agent SBR were determined to assist with the experimental design of future imaging studies. Approach: Following illumination with 670, 760, or 808 nm, autofluorescence in the NIR-I ( Results: Mice fed chow exhibit high levels of background autofluorescence in the gastrointestinal tract and, to a lesser extent, skin when illuminated with 670 nm light for NIR-I imaging (700 to 975 nm), interfering with the identification of fluorescently labeled tissue. Background autofluorescence was reduced by more than two orders of magnitude by any of the following changes: (1) purified diet; (2) excitation with 760 or 808 nm illumination; or (3) emission in the NIR-II (1000 to 1600 or 1250 to 1600 nm). Although the SBR was generally sufficient for feature identification except when imaging of chow-fed mice with 670 nm excitation and NIR-I emission, switching to a purified diet, using longer excitation wavelengths, or using longer emission wavelengths improved SBR significantly. Conclusions: Systematic comparison of imaging conditions and diet highlights the reduction in autofluorescence and increase in SBR enabled by intentional choices in the experimental parameters including diet, excitation wavelength, and emission wavelength range.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.