Evidence map›Paper›PMID 37034995›Full record

ArticleiScience2023

Addition of dNTPs can improve the detection sensitivity of catalytic hairpin assembly.

Mingyuan Zou, Meiling Zhou, Shuo Ma, Chen Zhang, Feng Xiao, Huina Wu, Abudushalamu GuliNaizhaer, Yuming Yao, Yaya Chen, Shijie Cai and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mingyuan ZouSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Meiling ZhouSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Shuo MaSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Chen ZhangSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Feng XiaoSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Huina WuSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Abudushalamu GuliNaizhaerSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Yuming YaoSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Yaya ChenSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Shijie CaiSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Xiaobo FanDepartment of Clinical Laboratory Medicine, Medical School, Southeast University, Nanjing 210009, People's Republic of China.
Fengfeng ZhaoSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.
Guoqiu WuSoutheast University, Zhongda Hospital, Center of Clinical Laboratory Medicine, Medical School, Nanjing 210009, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ever since the catalytic hairpin assembly (CHA) circuit has been highlighted as a powerful nucleic acid detection tool, nucleic acid detection methods based on CHA have been widely studied. However, the detection sensitivity of CHA-based methods is insufficient. The relatively high background signals resulting from the spontaneous reaction between the two hairpin probes is one of the major reasons for limiting the sensitivity of CHA. In this study, we established that the addition of deoxynucleotide triphosphates (dNTPs) to the reaction system can significantly reduce the background leakage of CHA. The dNTPs-CHA, coupled with a fluorescence lateral flow assay strip, is used for the rapid and highly sensitive detection of miRNA. It is capable of reliably detecting miRNA in serum samples down to a limit of 100 aM, which is an improvement in the lower detection limit by nearly five orders of magnitude compared to that of the pure CHA.

Indexed as

Biological sciencesDiagnosticsMolecular biology

Identifiers

PMID37034995
PMCPMC10074145

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.