Evidence map›Paper›PMID 37034749›Full record

ArticlebioRxiv : the preprint server for biology2023

Lysosome transporter purification and reconstitution identifies Ypq1 pH-gated lysine transport and regulation.

Felichi Mae Arines, Aleksander Wielenga, Olive E Burata, Francisco Narro Garcia, Randy B Stockbridge, Ming Li

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Felichi Mae ArinesDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Aleksander WielengaDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Olive E BurataDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Francisco Narro GarciaDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Randy B StockbridgeDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Ming LiDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
University of Michigan · US

Funding

Structural and functional analysis of novel microbial membrane proteinsR35GM128768 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Randy B. Stockbridge · 2018 to 2026
$3.7M
Undergrad supplement: Ubiquitin-Dependent Protein Regulation and Quality Control of the Lysosomal MembraneR01GM133873 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LI, MING · 2019 to 2023
$1.9M
NIGMS NIH HHS R01 GM133873NIGMS NIH HHS R35 GM128768
6 · The paper itself

Abstract

Lysosomes achieve their function through numerous transporters that import or export nutrients across their membrane. However, technical challenges in membrane protein overexpression, purification, and reconstitution hinder our understanding of lysosome transporter function. Here, we developed a platform to overexpress and purify the putative lysine transporter Ypq1 using a constitutive overexpression system in protease- and ubiquitination-deficient yeast vacuoles. Using this method, we purified and reconstituted Ypq1 into proteoliposomes and showed lysine transport function, supporting its role as a basic amino acid transporter on the vacuole membrane. We also found that the absence of lysine destabilizes purified Ypq1 and causes it to aggregate, consistent with its propensity to be downregulated

Indexed as

membrane proteinproteoliposomepurification and reconstitutiontransporter assayVacuole

Identifiers

PMID37034749
PMCPMC10081341
OpenAlexW4362509786

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.